EMCS (N-ε-malemidocaproyl-oxysuccinimide ester)

Citas y referencias (7)

Thermo Scientific™

EMCS (N-ε-malemidocaproyl-oxysuccinimide ester)

Thermo Scientific Pierce EMCS es un entrecruzador de amina a sulfhidrilo que contiene grupos reactivos de éster NHS y maleimidaMás información

Número de catálogo	Cantidad
22308	50 mg

Número de catálogo 22308

Precio (MXN)

Cantidad:

50 mg

Pedido a granel o personalizado

Thermo Scientific Pierce EMCS es un entrecruzador de amina a sulfhidrilo que contiene grupos reactivos de éster NHS y maleimida en los extremos opuestos de un brazo espaciador de longitud media (9,4 angstroms).

Características de EMCS:

• Grupos reactivos: Éster NHS y maleimida
• Reactivo hacia: grupos amino y sulfhidrilo
• C6-homólogo de series de espaciadores alifáticos que incluye AMAS, BMPS, GMBS y EMCS
• El extremo de éster NHS se une con aminas primarias a pH 7-9 para formar enlaces de amida estables
• Las maleimidas reaccionan con grupos -SH a pH 6,5-7,5, formando enlaces de tioéter estables
• No escindible
• Insoluble en agua (disolver primero en DMF o DMSO); comparar con Sulfo-EMCS
• Esfera aumentada de acoplamiento vs. GMBS
• El espaciador corto alifático tiene un bajo potencial para obtener una respuesta inmunitaria

Referencias del producto:
Guía de aplicación entrecruzada: búsqueda de referencias literarias recientes para este producto

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Permeabilidad celularSí

DescripciónEMCS

FormularioPolvo

Método de etiquetadoEtiqueta química

Peso molecular308.29

PegiladoNo

Línea de productosPierce

Cantidad50 mg

Fracción reactivaMaleimida, Éster NHS

Condiciones de envíoAmbiente

SolubilidadDMF, DMSO

Longitud del brazo del separador9,4 Å

Soluble en aguaNo

Reactividad químicaAmino-sulfhidrilo

CleavableNo

Tipo de enlace cruzadoHeterobifuncionales

FormatoEstándar

Tipo de productoEntrecruzador

SeparadorCorto (< 10 Å)

Unit SizeEach

Contenido y almacenamiento

Tras su recepción, almacenar desecado a 4 °C.

Preguntas frecuentes

Can you provide the shelf-life for EMCS (N-ε-malemidocaproyl-oxysuccinimide ester)?

EMCS (N-ε-malemidocaproyl-oxysuccinimide ester) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Citations & References (7)

Citations & References

Abstract

Mechanism of reversal of phospholamban inhibition of the cardiac Ca2+-ATPase by protein kinase A and by anti-phospholamban monoclonal antibody 2D12.

Authors:Chen Z, Akin BL, Jones LR

Journal:J Biol Chem

PubMed ID:17548345

'Our model of phospholamban (PLB) regulation of the cardiac Ca(2+)-ATPase in sarcoplasmic reticulum (SERCA2a) states that PLB binds to the Ca(2+)-free, E2 conformation of SERCA2a and blocks it from transitioning from E2 to E1, the Ca(2+)-bound state. PLB and Ca(2+) binding to SERCA2a are mutually exclusive, and PLB inhibition of ... More

Miniature single-particle immunoassay for prostate-specific antigen in serum using recombinant Fab fragments.

Authors:Härmä H, Tarkkinen P, Soukka T, Lövgren T

Journal:Clin Chem

PubMed ID:11067810

Quantitative, miniaturized nucleic acid assays and immunoassays can be developed with single microparticles, microfluorometric detection, and intrinsically fluorescent lanthanide chelates in a multiple assay format to decrease reagent consumption, cost, and assay time. We used recombinant Fab fragments to capture and detect free and total prostate-specific antigen (PSA) from serum ... More

Characterizing phospholamban to sarco(endo)plasmic reticulum Ca2+-ATPase 2a (SERCA2a) protein binding interactions in human cardiac sarcoplasmic reticulum vesicles using chemical cross-linking.

Authors:Akin BL, Jones LR

Journal:J Biol Chem

PubMed ID:22247554

Chemical cross-linking was used to study protein binding interactions between native phospholamban (PLB) and SERCA2a in sarcoplasmic reticulum (SR) vesicles prepared from normal and failed human hearts. Lys(27) of PLB was cross-linked to the Ca(2+) pump at the cytoplasmic extension of M4 (at or near Lys(328)) with the homobifunctional cross-linker, ... More

PNA microarrays for hybridisation of unlabelled DNA samples.

Authors:Brandt O, Feldner J, Stephan A, Schröder M, Schnölzer M, Arlinghaus HF, Hoheisel JD, Jacob A

Journal:Nucleic Acids Res

PubMed ID:14500847

Several strategies have been developed for the production of peptide nucleic acid (PNA) microarrays by parallel probe synthesis and selective coupling of full-length molecules. Such microarrays were used for direct detection of the hybridisation of unlabelled DNA by time-of-flight secondary ion mass spectrometry. PNAs were synthesised by an automated process ... More

Role of leucine 31 of phospholamban in structural and functional interactions with the Ca2+ pump of cardiac sarcoplasmic reticulum.

Authors:Chen Z, Stokes DL, Jones LR

Journal:J Biol Chem

PubMed ID:15644311

The ability of two loss-of-function mutants, L31A and L31C, of phospholamban (PLB) to bind to and inhibit the Ca(2+) pump of cardiac sarcoplasmic reticulum (SERCA2a) was investigated using a molecular cross-linking approach. Leu(31) of PLB, located at the cytoplasmic membrane boundary, is a critical amino acid shown previously to be ... More

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