DTME (dithiobismaleimidoethane)

Citas y referencias (5)

Thermo Scientific™

DTME (dithiobismaleimidoethane)

Thermo Scientific Pierce DTME es un entrecruzador de maleimida de longitud media para la conjugación covalente reversible entre grupos sulfhidriloMás información

Número de catálogo	Cantidad
22335	50 mg

Número de catálogo 22335

Precio (MXN)

Cantidad:

50 mg

Pedido a granel o personalizado

Thermo Scientific Pierce DTME es un entrecruzador de maleimida de longitud media para la conjugación covalente reversible entre grupos sulfhidrilo (por ejemplo, cisteínas de proteínas o péptidos) mediante la reducción del enlace disulfuro en el centro del brazo espaciador.

Características de DTME:

• Grupos reactivos: maleimida (ambos extremos)
• Reactiva hacia: grupos sulfhidrilo
• Entrecruzador de sulfhidrilo a sulfhidrilo largo (13,3A) no escindible, compuesto por grupos de maleimida y brazo espaciador de disulfuro de 11 átomos
• Insoluble al agua: disolver primero en DMF o DMSO y añadir a los tampones de reacción acuosa
• Escindible por reducción de brazo espaciador con disulfuro con DTT, TCEEP u otro agente reductor

Referencias del producto
Guía de aplicación entrecruzada: búsqueda de referencias literarias recientes para este producto

Productos relacionados
BMH (bismaleimidohexano)

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Permeabilidad celularSí

DescripciónDTME

FormularioSólido

Método de etiquetadoEtiqueta química

Peso molecular312.36

PegiladoNo

Línea de productosPierce

Cantidad50 mg

Fracción reactivaMaleimida

Condiciones de envíoAmbiente

SolubilidadDMF, DMSO

Longitud del brazo del separador13,3 Å

Soluble en aguaNo

Reactividad químicaSulfhidrilo-sulfhidrilo

CleavablePor tioles

Tipo de enlace cruzadoHomobifuncionales

FormatoEstándar

Tipo de productoEntrecruzador

SeparadorMedio (de 10 a 30 Å)

Unit SizeEach

Contenido y almacenamiento

Tras su recepción, almacenar desecado a 4 °C.

Preguntas frecuentes

Can you provide the shelf-life for DTME (dithiobismaleimidoethane)?

DTME (dithiobismaleimidoethane) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Citations & References (5)

Citations & References

Abstract

Nedd4-1 and beta-arrestin-1 are key regulators of Na+/H+ exchanger 1 ubiquitylation, endocytosis, and function.

Authors:Simonin A, Fuster D

Journal:J Biol Chem

PubMed ID:20855896

'The ubiquitously expressed mammalian Na(+)/H(+) exchanger 1 (NHE1) controls cell volume and pH but is also critically involved in complex biological processes like cell adhesion, cell migration, cell proliferation, and mechanosensation. Pathways controlling NHE1 turnover at the plasma membrane, however, are currently unclear. Here, we demonstrate that NHE1 undergoes ubiquitylation ... More

The extreme C terminus of the ABC protein DrrA contains unique motifs involved in function and assembly of the DrrAB complex.

Authors:Zhang H, Pradhan P, Kaur P

Journal:J Biol Chem

PubMed ID:20876527

'Two novel regulatory motifs, LDEVFL and C-terminal regulatory Glu (E)-rich motif (CREEM), are identified in the extreme C terminus of the ABC protein DrrA, which is involved in direct interaction with the N-terminal cytoplasmic tail of the membrane protein DrrB and in homodimerization of DrrA. Disulfide cross-linking analysis showed that ... More

Association of Rho-associated protein kinase 1 with E-cadherin complexes is mediated by p120-catenin.

Authors:Smith AL, Dohn MR, Brown MV, Reynolds AB

Journal:Mol Biol Cell

PubMed ID:22031287

The dynamic functional linkage of cadherins with the underlying actin cytoskeleton is tightly regulated to achieve proper cell-cell adhesion. p120-catenin (p120) regulates both cadherin stability and actin dynamics, but the relationship between these two functions remains unclear. Using a novel proteomic approach called reversible cross-link immunoprecipitation, or ReCLIP, we previously ... More

Claudin-2 forms homodimers and is a component of a high molecular weight protein complex.

Authors:Van Itallie CM, Mitic LL, Anderson JM

Journal:J Biol Chem

PubMed ID:21098027

Tight junctions are multiprotein complexes that form the fundamental physiologic and anatomic barrier between epithelial and endothelial cells, yet little information is available about their molecular organization. To begin to understand how the transmembrane proteins of the tight junction are organized into multiprotein complexes, we used blue native-PAGE (BN-PAGE) and ... More

Strategies for stabilizing superoxide dismutase (SOD1), the protein destabilized in the most common form of familial amyotrophic lateral sclerosis.

Authors:Auclair JR, Boggio KJ, Petsko GA, Ringe D, Agar JN

Journal:Proc Natl Acad Sci U S A

PubMed ID:21098299

Amyotrophic lateral sclerosis (ALS) is a disorder characterized by the death of both upper and lower motor neurons and by 3- to 5-yr median survival postdiagnosis. The only US Food and Drug Administration-approved drug for the treatment of ALS, Riluzole, has at best, moderate effect on patient survival and quality ... More