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인용 및 참조 문헌 (5)
Thermo Scientific™
DTME (dithiobismaleimidoethane)
Thermo Scientific Pierce DTME is a mid-length, maleimide crosslinker for reversible covalent conjugation between sulfhydryl groups (e.g., protein or peptide자세히 알아보기
| 카탈로그 번호 | 수량 |
|---|---|
| 22335 | 50 mg |
카탈로그 번호 22335
제품 가격(KRW)
459,000
온라인 행사
Ends: 31-Mar-2026
539,000할인액 80,000 (15%)
Each
수량:
50 mg
제품 가격(KRW)
459,000
온라인 행사
Ends: 31-Mar-2026
539,000할인액 80,000 (15%)
Each
Thermo Scientific Pierce DTME is a mid-length, maleimide crosslinker for reversible covalent conjugation between sulfhydryl groups (e.g., protein or peptide cysteines) by reduction of the disulfide bond in the center of the spacer arm.
Features of DTME:
• Reactive groups: maleimide (both ends)
• Reactive towards: sulfhydryl groups
• Long (13.3A), cleavable, sulfhydryl-to-sulfhydryl crosslinker, composed of maleimide groups and 11-atom disulfide spacer arm
• Water-insoluble—dissolve first in DMF or DMSO, then add to aqueous reaction buffers
• Cleavable by reduction of disulfide spacer arm with DTT, TCEP or other reducing agent
Product References:
Crosslinker Application Guide -- search for recent literature references for this product
Related Products
BMH (bismaleimidohexane)
Features of DTME:
• Reactive groups: maleimide (both ends)
• Reactive towards: sulfhydryl groups
• Long (13.3A), cleavable, sulfhydryl-to-sulfhydryl crosslinker, composed of maleimide groups and 11-atom disulfide spacer arm
• Water-insoluble—dissolve first in DMF or DMSO, then add to aqueous reaction buffers
• Cleavable by reduction of disulfide spacer arm with DTT, TCEP or other reducing agent
Product References:
Crosslinker Application Guide -- search for recent literature references for this product
Related Products
BMH (bismaleimidohexane)
For Research Use Only. Not for use in diagnostic procedures.
사양
세포 투과성Yes
설명DTME
형태Solid
라벨링 방법Chemical Labeling
분자량312.36
페길레이티드No
제품라인Pierce
수량50 mg
반응성 부분Maleimide
배송 조건Ambient
용해도DMF, DMSO
스페이서 암 길이13.3 Å
수용성No
화학물질 반응성Sulfhydryl-Sulfhydryl
CleavableBy Thiols
교차결합제 유형Homobifunctional
형식Standard
제품 유형Crosslinker
스페이서Medium (10 to 30 Å)
Unit SizeEach
구성 및 보관
Upon receipt store desiccated at 4°C.
자주 묻는 질문(FAQ)
Can you provide the shelf-life for DTME (dithiobismaleimidoethane)?
인용 및 참조 문헌 (5)
인용 및 참조 문헌
Abstract
Nedd4-1 and beta-arrestin-1 are key regulators of Na+/H+ exchanger 1 ubiquitylation, endocytosis, and function.
Journal:J Biol Chem
PubMed ID:20855896
'The ubiquitously expressed mammalian Na(+)/H(+) exchanger 1 (NHE1) controls cell volume and pH but is also critically involved in complex biological processes like cell adhesion, cell migration, cell proliferation, and mechanosensation. Pathways controlling NHE1 turnover at the plasma membrane, however, are currently unclear. Here, we demonstrate that NHE1 undergoes ubiquitylation
The extreme C terminus of the ABC protein DrrA contains unique motifs involved in function and assembly of the DrrAB complex.
Journal:J Biol Chem
PubMed ID:20876527
'Two novel regulatory motifs, LDEVFL and C-terminal regulatory Glu (E)-rich motif (CREEM), are identified in the extreme C terminus of the ABC protein DrrA, which is involved in direct interaction with the N-terminal cytoplasmic tail of the membrane protein DrrB and in homodimerization of DrrA. Disulfide cross-linking analysis showed that
Association of Rho-associated protein kinase 1 with E-cadherin complexes is mediated by p120-catenin.
Journal:Mol Biol Cell
PubMed ID:22031287
The dynamic functional linkage of cadherins with the underlying actin cytoskeleton is tightly regulated to achieve proper cell-cell adhesion. p120-catenin (p120) regulates both cadherin stability and actin dynamics, but the relationship between these two functions remains unclear. Using a novel proteomic approach called reversible cross-link immunoprecipitation, or ReCLIP, we previously
Claudin-2 forms homodimers and is a component of a high molecular weight protein complex.
Journal:J Biol Chem
PubMed ID:21098027
Tight junctions are multiprotein complexes that form the fundamental physiologic and anatomic barrier between epithelial and endothelial cells, yet little information is available about their molecular organization. To begin to understand how the transmembrane proteins of the tight junction are organized into multiprotein complexes, we used blue native-PAGE (BN-PAGE) and
Strategies for stabilizing superoxide dismutase (SOD1), the protein destabilized in the most common form of familial amyotrophic lateral sclerosis.
Journal:Proc Natl Acad Sci U S A
PubMed ID:21098299
Amyotrophic lateral sclerosis (ALS) is a disorder characterized by the death of both upper and lower motor neurons and by 3- to 5-yr median survival postdiagnosis. The only US Food and Drug Administration-approved drug for the treatment of ALS, Riluzole, has at best, moderate effect on patient survival and quality