Medio de suero reducido Opti-MEM™, polvo

Name: Medio de suero reducido Opti-MEM™, polvo
SKU: 22600134

Opti-MEM™ Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementationMás información

Cambiar vista

Número de catálogo	Cantidad
22600134	10 L
22600050	10 x1 L

2 opciones

Número de catálogo 22600134

Precio (MXN)

Cantidad:

10 L

Customize this product

Opti-MEM™ Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change to growth rate or morphology. Opti-MEM™ is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM™ can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. We offer a variety of Gibco™ Opti-MEM™ modifications for a range of cell culture applications.

This Opti-MEM™ is modified as follows:

With

• L-glutamine

• Phenol Red

The complete formulation is confidential. For more information, please contact Technical Services.

Gibco™ Opti-MEM™ is a unique media that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%.

Gibco™ Opti-MEM™ uses a sodium bicarbonate buffer system (2.4 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment and filtration at the time of preparation (see protocol for details).

no está indicado para uso terapéutico en humanos ni en animales. Los usos que vayan más allá del uso previsto podrían infringir la legislación local. Para uso diagnóstico in vitro.

Especificaciones

Línea de célulasSp2, AE-1, CHO, BHK-21 y HEK

Tipo de célulaFibroblastos primarios

Calidad de fabricacióncGMP-compliant under the ISO 13485 standard

Línea de productosGibco, Opti-MEM

Tipo de productoOpti-MEM I

Cantidad10 L

Duración de almacenamiento30 meses a partir de la fecha de fabricación

Condiciones de envíoTemperatura ambiente

ClasificaciónOrigen animal

FormularioPolvo

EsterilidadEstéril con filtro

Con aditivosGlutamina, Rojo de fenol

Unit SizeEach

Contenido y almacenamiento

Condiciones de almacenamiento: De 2 a 8 °C
Condiciones de envío: Ambiente
Vida útil: 30 meses a partir de la fecha de fabricación

Preguntas frecuentes

Do you offer an alternative for Opti-MEM Reduced Serum Medium powder (Cat. No. 22600050)?

The direct alternative for Opti-MEM Reduced Serum Medium powder (Cat. No. 22600050) is Opti-MEM Reduced Serum Medium powder (Cat. No. 22600134). The product is the same but comes in a 10 L volume. Therefore, you might consider reconstituting the powder and then aliquoting the resulting media into smaller bottles.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How much Opti-MEM Reduced Serum Medium, powder (Cat. No. 22600050, 22600134) should I use to reconstitute the media?

We recommend reconstituting Opti-MEM Reduced Serum Medium, powder (Cat. No. 22600050, 22600134) at 13.592 g/L.

Is there any other difference between Opti-MEM I Reduced Serum Medium and Opti-MEM I Reduced Serum Medium, no phenol red besides the presence/absence of phenol red?

The formulation of Opti-MEM I Reduced Serum Medium and that of Opti-MEM I Reduced Serum Medium, no phenol red is the same except for the presence of phenol red in the former medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Where can I find the osmolality for my lot of Opti-MEM I Reduced Serum Medium?

We do provide osmolality information on the certificate of analysis. All lots of Opti-MEM I Reduced Serum Medium will meet the osmolality specification of ≥280 to ≤320 mOsm/kg.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I aliquot and freeze Opti-MEM I Reduced Serum Medium to conserve it longer? If so, for how long can it be stored?

Sorry, we don't have data to show that frozen Opti-MEM I Reduced Serum Medium has a longer shelf life. Actually, we recommend storing this medium at 2-8 degrees C in the dark. We do not recommend freezing the medium because it may precipitate.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Medio de suero reducido Opti-MEM™, polvo FAQs