SPB (succinimidyl-[4-(psoralen-8-yloxy)]-butyrate)
SPB (succinimidyl-[4-(psoralen-8-yloxy)]-butyrate)
Thermo Scientific™

SPB (succinimidyl-[4-(psoralen-8-yloxy)]-butyrate)

Thermo Scientific Pierce SPB es un entrecruzador de éster NHS y psoraleno que conjuga eficazmente aminas primarias con ADN medianteMás información
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Número de catálogoCantidad
2301350 mg
Número de catálogo 23013
Precio (MXN)
-
Cantidad:
50 mg
Thermo Scientific Pierce SPB es un entrecruzador de éster NHS y psoraleno que conjuga eficazmente aminas primarias con ADN mediante intercalación que se activa con luz junto a bases pirimidínicas.

Características de SPB:

• Grupos reactivos: Éster NHS y psoraleno
• Reactivo hacia: grupos aminos y timina
• Agente de ADN intercalante y sonda de ácido nucleico1,2
• Reactivo para fotoinmovilización a microplacas3
• Punto de fusión: 177-178 °C

Referencias del producto:
Guía de aplicación entrecruzada: búsqueda de referencias literarias recientes para este producto
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Permeabilidad celularNo determinado
DescripciónSPB
FormularioSólido
Método de etiquetadoEtiqueta química
Peso molecular385.32
PegiladoNo
Línea de productosPierce
Cantidad50 mg
Fracción reactivaPsoraleno, éster NHS
Condiciones de envíoAmbiente
SolubilidadDMF, DMSO
Longitud del brazo del separador8,6 Å
Soluble en aguaNo
Reactividad químicaAmina no selectiva
CleavableNo
Tipo de enlace cruzadoHeterobifuncionales
FormatoEstándar
Tipo de productoEntrecruzador
SeparadorCorto (< 10 Å)
Unit SizeEach
Contenido y almacenamiento
Tras su recepción, almacenar desecado a 4 °C.

Preguntas frecuentes

Can you provide the shelf-life for SPB (succinimidyl-[4-(psoralen-8-yloxy)]-butyrate)?

SPB (succinimidyl-[4-(psoralen-8-yloxy)]-butyrate) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What are the options for crosslinking proteins to nucleic acids, and what are the differences between them?

We offer the following crosslinkers for protein to nucleic acids: formaldehyde (Cat. No. 28906), SPB (Cat. No. 23013), SDAD (Cat. No. 26169), sulfo-SDAD (Cat. No. 26175), and sulfo-SBED (Cat. No. A39260).
Formaldehyde can be used for DNA-DNA crosslinking as well as DNA-protein crosslinking. While it is good for crosslinking proteins that are in direct contact with DNA, formaldehyde cannot link proteins that may be bound in a complex with other proteins but are not in direct contact with DNA. EGS or DSG can crosslink proteins to other proteins that are in direct contact with DNA and crosslinked to DNA by formaldehyde.
SPB, Sulfo-SDAD, and Sulfo-SBED are all photoreactive crosslinkers. SPB is an NHS-ester and psoralen heterobifunctional crosslinker that conjugates primary amines on proteins to DNA via photo-activated intercalation of psoralen to pyrimidine bases. The psoralen tricyclic planar ring system intercalates into double-stranded, and to a lesser extent, single-stranded DNA and RNA. The photoreactive psoralen group provides more selective covalent binding to nucleic acids than either phenyl azide or diazerine linkers, which makes SPB the best choice. The diazerine on sulfo-SDAD and the phenyl azide on sulfo-SBED are not selective for nucleic acids and can crosslink other biomolecules in the same besides any protein-DNA interactions. Sulfo-SBED involves biotin transfer rather than actual linking.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.