StemPro® EZChek™ Human Tri-Lineage Multiplex PCR Kit

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The StemPro® EZChek™ Human Tri-Lineage Multiplex PCR Kit uses one pluripotency marker, three early differentiation markers and a GAPDH control to rapidly and reliably monitor the differentiation state of human embryonic stem cells (hESCs), human induced pluripotent stem cells (iPSCs) and pluripotent human embryonal carcinoma stem cells (hECs) using a convenient, one tube, multiplex RT-PCR assay. Cultures can be tested for differentiation state (are the cultures undifferentiated) and differentiation potential (can the cultures be induced to form the three embryonic lineages). This kit offers the benefit of being able to perform PCR with much smaller sample volumes and contains primers for detecting the following five human genes in one multiplex PCR reaction rather than having to perform five individual PCR reactions:

–Pou5f1⁄Oct4: marker for the pluripotent state of hESCs and hECs
–AFP: endoderm lineage marker

–ACTC1: mesoderm lineage marker
–SOX1: ectoderm lineage marker
–GAPDH: internal RNA standard for normalizing mRNA levels

Additional component required for complete kit (need to be purchased separately):
AccuPrime™ Pfx SuperMix (Cat. no. 12344-040)

Intended use(s):
Research use only (RUO).
Caution: not intended for human or animal diagnostic or therapeutic uses.
For human ex vivo tissue and cell culture processing applications. CAUTION: When used as a medical device, Federal Law restricts this device to sale by or on the order of a physician.


PCR Method: 2-Step RT-PCR
Reverse Transcriptase: SuperScript® III
Polymerase: AccuPrime™ Pfx SuperMix (Not Included)
Hot Start: Built-In Hot Start
Sample Type (General): RNA
Target Gene: GAPDH, AFP, ACTC1, SOX1, Pou5f1⁄Oct4
Product Size: 100 reactions
Detection Method: Gel Electrophoresis
Target Specificity: Target-Specific
Primer-Probe Compatibility: Unlabeled Oligos

Contents & storage

STEMPRO® EZChek™ Human Tri-Lineage Multiplex PCR Kit includes the following components (enough for 100 reactions):

–STEMPRO® EZChek™ Human Tri-Lineage Primer Mix: Store at -20°C (non-frost-free).
–SuperScript® III First-Strand Synthesis SuperMix: Store at -20°C (non-frost-free).

– PureLink™ Micro-to-Midi™ Total RNA Purification System: Store at Room Temperature.

This product (Cat. no. 23191-050) is shipped in two parts with separate storage requirements and catalog numbers:

1. STEMPRO® EZChek™ Human Tri-Lineage Primer Mix (100 reactions, Cat. no. A10250) and
SuperScript® III First-Strand Synthesis SuperMix (2 x 50 reactions, 2 x Cat. no. 18080-400).
2. PureLink™ Micro-to-Midi™ Total RNA Purification System (100 reactions, Cat. no. 45-6001).

These component items (with the exception of SuperScript® III First-Strand Synthesis SuperMix) are not available for purchase separately.


Manuals & protocols