Thermo Scientific™

Pierce™ BCA Protein Assay Kit - Reducing Agent Compatible

Catalog number:  23250

 Related applications:

Protein Assays and Analysis

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This BCA Protein Assay Kit is the reducing agent-compatible version of our popular Thermo Scientific Pierce BCA Protein Assay. The kit enables you to measure protein concentration in samples that contain thiol-reductants dithiothreitol (DTT) and 2-mercaptoethanol (BME).

Features of the BCA Protein Assay Kit—Reducing Agent Compatible:

Compatible—assay samples that contain up to 5 mM DTT, 35 mM BME, or 10 mM TCEP
BCA technology—only a slight modification of the standard BCA Protein Assay protocol (15-minute incubation with Compatibility Reagent); no precipitation steps required
Small samples—requires only 25 µL (standard kit) or less than 10 µL (microplate kit) of sample
Colorimetric—measure with a standard spectrophotometer or plate reader (562nm)
Excellent uniformity—exhibits less protein-to-protein variation than dye-binding methods
High linearity—linear working range for BSA equals 125 to 2000 µg/mL

The BCA Protein Assay Kit—Reducing Agent Compatible (BCA-RAC) provides all of the advantages of the original BCA Assay, plus compatibility with disulfide reducing agents at concentrations routinely used in protein sample buffers. This special adaptation of the popular Pierce BCA Protein Assay method enables accurate protein concentration measurement for samples containing DTT, 2-ME or TCEP. This reducing agent compatible (RAC) BCA Kit extends the already broad reagent compatibility of the BCA Protein Assay protocol to include nearly all types of components commonly present in protein research samples.

Related Products
Pierce™ Microplate BCA Protein Assay Kit - Reducing Agent Compatible
96-Well Plates for Pierce™ BCA-RAC Assay
For Research Use Only. Not for use in diagnostic procedures.


Assay Range: 125-2,000µg/mL
Assay Type: Protein Quantitation Assay
Detection Method: Colorimetric (562 nm)
For Use With (Equipment): Microplate Reader, Spectrophotometer
Product Line: Pierce™
Product Size: 275 mL
Technique: Absorbance, Solution-based Detection

Contents & storage

Sufficient For: 250 tube assays with 25 µL samples
• BCA Reagent A, 250 mL
• BCA Reagent B, 25 mL
• Compatibility Reagent, 10 x 20 mg
• Reconstitution Buffer, 15 mL
• Albumin Standard Ampules, 2mg/mL, 10 x 1 mL

Store at room temperature


Manuals & protocols