Thermo Scientific™

Pierce™ Quantitative Peptide Assays & Standards

Catalog number: 23290
Thermo Scientific™

Pierce™ Quantitative Peptide Assays & Standards

Catalog number: 23290
Pierce colorimetric or fluorescent quantitative peptide assays are easy mix-and-read microplate assays with stable signals for accurate and robust measurement of peptide digest samples. A high-quality peptide digest reference standard is provided as a stand-alone, or in the kits for use in the generation of linear standard curves for improved accuracy and reproducibility. The increased sensitivity, low sample assay volume, and included reference standard are essential for accurate and robust measurement of peptide digest samples for normalization of sample injection amount for MS analysis.
 
Catalog Number
23290
Unit Size
500 assays
Description
Quantitative Fluorometric Peptide Assay
Price (USD)
Price: 448.00
Your Price:
Availability
***
Full specifications
Quantity
DescriptionQuantitative Fluorometric Peptide Assay
For Use With (Equipment)Microplate Reader
Product TypePeptide Assay
Chemical ReactivityAmine
Product LinePierce™
Starting MaterialPeptides, Protease-digested Protein
Final Product TypePeptides
Detection MethodFluorescent
FormatKit
Workflow StepIn-Process Testing
Quantity500 Assays
Unit Size500 assays
Catalog Number
Unit Size
500 assays
Description
Quantitative Colorimetric Peptide Assay
Price (USD)
Price: 412.00
Your Price:
Availability
***
Full specifications
Quantity
DescriptionQuantitative Colorimetric Peptide Assay
For Use With (Equipment)Microplate Reader
Product TypePeptide Assay
Product LinePierce™
Starting MaterialPeptides, Protease-digested Protein
Final Product TypePeptides
Detection MethodColorimetric
FormatKit
Workflow StepIn-Process Testing
Quantity500 Assays
Unit Size500 assays
Catalog Number
Unit Size
1.5 mL
Description
Peptide Digest Assay Standard (1 mg/mL)
Price (USD)
Price: 164.00
Your Price:
Availability
***
Full specifications
Quantity
DescriptionPeptide Digest Assay Standard (1 mg/mL)
For Use With (Equipment)Microplate Reader
Product TypePeptide Digest Assay Standard
Product LinePierce™
Starting MaterialPeptides, Protease-digested Protein
Detection MethodColorimetric, Fluorescent
FormatLiquid
Workflow StepIn-Process Testing
Quantity1.5 mL
Unit Size1.5 mL
Showing 3 of 3
Catalog NumberSpecificationsUnit SizeDescriptionPrice (USD)AvailabilityQuantity
23290Full specifications
500 assaysQuantitative Fluorometric Peptide Assay
Price: 448.00
Your Price:
***
DescriptionQuantitative Fluorometric Peptide Assay
For Use With (Equipment)Microplate Reader
Product TypePeptide Assay
Chemical ReactivityAmine
Product LinePierce™
Starting MaterialPeptides, Protease-digested Protein
Final Product TypePeptides
Detection MethodFluorescent
FormatKit
Workflow StepIn-Process Testing
Quantity500 Assays
Unit Size500 assays
23275Full specifications
500 assaysQuantitative Colorimetric Peptide Assay
Price: 412.00
Your Price:
***
DescriptionQuantitative Colorimetric Peptide Assay
For Use With (Equipment)Microplate Reader
Product TypePeptide Assay
Product LinePierce™
Starting MaterialPeptides, Protease-digested Protein
Final Product TypePeptides
Detection MethodColorimetric
FormatKit
Workflow StepIn-Process Testing
Quantity500 Assays
Unit Size500 assays
23295Full specifications
1.5 mLPeptide Digest Assay Standard (1 mg/mL)
Price: 164.00
Your Price:
***
DescriptionPeptide Digest Assay Standard (1 mg/mL)
For Use With (Equipment)Microplate Reader
Product TypePeptide Digest Assay Standard
Product LinePierce™
Starting MaterialPeptides, Protease-digested Protein
Detection MethodColorimetric, Fluorescent
FormatLiquid
Workflow StepIn-Process Testing
Quantity1.5 mL
Unit Size1.5 mL
Showing 3 of 3
Sensitive—accurately detect as little as 5 µg/mL (fluorometric) or 25 µg/mL (colorimetric) of peptide mixture
Reproducible—assay performance rigorously tested using peptide digest mixtures
Robust peptide digest standard—kit includes a validated peptide digest standard for improved reproducibility of quantitation
Compatible—works with many reagents, including MS sample preparation reagents and TMT-labeled peptides (fluorometric assay)
Convenient—easy, mix-and-read format with stable signal that may be read in as little as 5 minutes (fluorometric) or 15 minutes (colorimetric)

Pierce Quantitative Colorimetric Peptide Assay provides modified BCA reagents for the reduction of Cu+2 to Cu+1, a proprietary chelator optimized for the quantitation of peptide mixtures, and peptide digest reference standard for use in the generation of control linear standard curves. This colorimetric peptide assay requires a small amount of sample (20 µL) and has a working peptide concentration range of 25–1000 μg/mL. In the reaction, the copper is first reduced by the amide backbone of peptides under alkaline conditions (Biuret reaction), followed by the chelator coupling with the reduced copper to form a bright red complex (absorbs at 480 nm) that can be read in as little as 15 minutes. The signal produced from this reaction is 3–4 fold more sensitive than the Micro-BCA Protein Assay for peptide analysis. Colorimetric peptide assay is compatible with TMT-labeled peptides but is not recommended for synthetic peptides because the assay is affected by peptide amino acid content.

Pierce Quantitative Fluorescent Peptide Assay reagents include peptide assay buffer, fluorescent peptide labeling reagent, and a peptide digest assay standard for the quantitative measurement of peptide concentrations. This sensitive assay requires only 10 µL of sample, produces a linear response with increasing peptide concentrations (5–1000 μg/mL), and results in a stable final fluorescence that can be detected in as little as 5 minutes. In this assay, peptides are specifically labeled at the amino-terminus using an amine-reactive fluorescent reagent that is non-fluorescent until reacted with tryptic peptides. The fluorescently labeled peptides are detected on a microplate reader (ex390/em475). This assay is suitable for the quantitative measurement of peptide digest mixtures and synthetic peptides with an unblocked amino terminus. Fluorescent assays are not compatible with TMT-labeled peptides.

Thermo Scientific Peptide Digest Assay Standard was designed as a reference standard for use with the Pierce quantitative fluorometric and colorimetric peptide assays to improve reproducibility and accuracy of peptide quantitation. The Peptide Digest Assay Standard is provided in ready-to-use liquid format at 1 mg/mL. The reference protein has been digested with MS-grade trypsin to minimize missed cleavages. To help ensure consistent performance, digestion efficiency of the protein is monitored to help ensure lot-to-lot consistency, and quality is assessed by comparison to a reference standard.

Applications
• Normalizing sample concentrations for quantitative downstream applications
• Normalizing sample loading volumes for LC, MS, and LC/MS applications
• Measuring recovery after peptide clean-up procedures

For Research Use Only. Not for use in diagnostic procedures.

Contents & Storage

Fluorometric Peptide Assay Reagent, 4 x 2.5 mL
Fluorometric Peptide Assay Buffer, 50 mL
Peptide Digest Assay Standard, 1.5 mL

Store in refrigerator (2–8°C).