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Imperial™ Protein Stain, 3 x 1 L - FAQs

Imperial™ Protein Stain, 3 x 1 L - FAQs

View additional product information for Imperial™ Protein Stain - FAQs (24615, 24617)

7 product FAQs found

I am seeing visible aggregates in my bottle of Imperial Protein Stain. Is this normal?

Imperial Protein Stain contains additives that help slow formation of dye-dye aggregates, which occur in all Coomassie dye-based protein staining reagents. If left undisturbed, the reagent may form visible aggregates that settle in the bottle; however, gentle mixing completely disperses these aggregates. To ensure that a homogeneous sample of the reagent is used, mix reagent before pouring or dispensing.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

After staining my protein gel with Imperial Protein Stain, I am not seeing any band development. What went wrong?

Here are possible causes and solutions:

- No protein was present in sample. Load a known amount of purified protein as a control.
- Insufficient amount of protein in sample. Load more total protein in gel.
- SDS not completely removed from gel. Wash gel more extensively before staining.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I stained my protein gel with Thermo Scientific Imperial Protein Stain and am getting very high background. Do you have any tips?

This is most likely due to SDS interference. We recommend washing the gel extensively before starting the staining procedure.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Is the Imperial Protein Stain compatible with mass spectrometry analysis?

Yes, here (https://tools.thermofisher.com/content/sfs/brochures/TR0050-Stained-gels-for-MS.pdf) is the procedure for processing gels stained with Imperial Stain, for mass spectrometry analysis.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What is the sensitivity of the Imperial Protein Stain?

With the enhanced protocol, the Imperial Protein Stain can detect less than 3 ng protein per band in 3 hours.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Does Imperial Protein Stain contain Coomassie G-250 or Coomassie R-250?

Imperial Protein Stain contains Coomassie R-250. The rest of the composition is proprietary.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How should I store the Thermo Scientific Imperial Protein Stain and what is its shelf life?

We recommend storing the Imperial Protein Stain at room temperature where it is stable for a year.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.