L-Glutamine (200 mM)
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Thermo Scientific™

L-Glutamine (200 mM)

Gibco™ L-glutamine is an amino acid required for cell culture. L-glutamine participates in the formation of purine and pyrimidine nucleotides,Read more
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Catalog NumberQuantity
25030024
also known as 25030-024
100 mL
2503003220 mL
25030123
also known as 25030-123
20 x 100 mL
Catalog number 25030024
also known as 25030-024
Price (EUR)
27,65
Online Exclusive
29,15
Save 1,50 (5%)
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Quantity:
100 mL
Customize this product
Price (EUR)
27,65
Online Exclusive
29,15
Save 1,50 (5%)
Each
Add to cart
Gibco™ L-glutamine is an amino acid required for cell culture. L-glutamine participates in the formation of purine and pyrimidine nucleotides, amino sugars, glutathione, L-glutamate and other amino acids, as well as in protein synthesis and glucose production. L-Glutamine, unlike most other amino acids, is not stable in solution. The rate at which this degradation proceeds is a function of time, temperature and pH. We also offer Gibco™ GlutaMAX™ supplement as a stable alternative to L-glutamine.

Gibco™ L-glutamine 200 mM Solution is a stock solution ready-to-use as a cell culture supplement. The optimal use concentration is dependent upon the cell type and medium used to culture the cells, but generally falls in the range of 2 – 6 mM.

Dual-site cGMP Manufacturing and Quality System
Gibco™ L-glutamine is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Gibco™ L-glutamine product made in our Grand Island facility (25030-164). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.
Specifications
Concentration or Composition (by Analyte or Components)100 X
GlutamineL-Glutamine
Green FeaturesSustainable packaging
Physical FormLiquid
Recommended StorageStorage conditions: -5°C to -20°C. Protect from light.
Shipping conditions: Dry ice
Shelf life: 18 months from date of manufacture
Shipping ConditionDry Ice
Quantity100 mL
pH6 to 8
Unit SizeEach

Frequently asked questions (FAQs)

What is your recommended method for thawing L-Glutamine before adding to cell culture media?

We recommend thawing at 2-8 degrees C and then warming at 37 degrees C until the material goes into solution. Mix it as it warms up to spend minimal time at elevated temperatures before you aliquot it.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Why did my 200 mM L-glutamine precipitate when I thawed it?

When L-glutamine is in a concentrated stock solution it easily precipitates when cooled. Warming the solution briefly in a 37 degrees C water bath with gentle swirling will dissolve the precipitate. Do not use the product unless the precipitate is fully dissolved.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations & References (5)

Citations & References
Abstract
FRET measurements of intracellular cAMP concentrations and cAMP analog permeability in intact cells.
Authors:Börner S, Schwede F, Schlipp A, Berisha F, Calebiro D, Lohse MJ, Nikolaev VO,
Journal:Nat Protoc
PubMed ID:21412271
'Real-time measurements of second messengers in living cells, such as cAMP, are usually performed by ratiometric fluorescence resonance energy transfer (FRET) imaging. However, correct calibration of FRET ratios, accurate calculations of absolute cAMP levels and actual permeabilities of different cAMP analogs have been challenging. Here we present a protocol that ... More
A critical role for choline kinase-alpha in the aggressiveness of bladder carcinomas.
Authors:Hernando E, Sarmentero-Estrada J, Koppie T, Belda-Iniesta C, Ramírez de Molina V, Cejas P, Ozu C, Le C, Sánchez JJ, González-Barón M, Koutcher J, Cordón-Cardó C, Bochner BH, Lacal JC, Ramírez de Molina A,
Journal:Oncogene
PubMed ID:19448670
'Bladder cancer is one of the most common causes of death in industrialized countries. New tumor markers and therapeutic approaches are still needed to improve the management of bladder cancer patients. Choline kinase-alpha (ChoKalpha) is a metabolic enzyme that has a role in cell proliferation and transformation. Inhibitors of ChoKalpha ... More
Fabrication of synthetic polymer coatings and their use in feeder-free culture of human embryonic stem cells.
Authors:Nandivada H, Villa-Diaz LG, O'Shea KS, Smith GD, Krebsbach PH, Lahann J,
Journal:Nat Protoc
PubMed ID:21720316
The culture of human embryonic stem (hES) cells in defined and xenogeneic-free conditions will contribute substantially to future biotechnological and medical applications. To achieve this goal, we developed the first fully defined synthetic polymer coating poly[2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl)ammonium hydroxide] (PMEDSAH) that sustains long-term growth of hES cells in different culture media. ... More
High concordance of drug-induced human hepatotoxicity with in vitro cytotoxicity measured in a novel cell-based model using high content screening.
Authors:O'Brien PJ, Irwin W, Diaz D, Howard-Cofield E, Krejsa CM, Slaughter MR, Gao B, Kaludercic N, Angeline A, Bernardi P, Brain P, Hougham C,
Journal:Arch Toxicol
PubMed ID:16598496
To develop and validate a practical, in vitro, cell-based model to assess human hepatotoxicity potential of drugs, we used the new technology of high content screening (HCS) and a novel combination of critical model features, including (1) use of live, human hepatocytes with drug metabolism capability, (2) preincubation of cells ... More
Protection of Primary Dopaminergic Midbrain Neurons by GPR139 Agonists Supports Different Mechanisms of MPP(+) and Rotenone Toxicity.
Authors:Bayer Andersen K, Leander Johansen J, Hentzer M, Smith GP, Dietz GP,
Journal:Front Cell Neurosci
PubMed ID:27445691
'The G-protein coupled receptor 139 (GPR139) is expressed specifically in the brain in areas of relevance for motor control. GPR139 function and signal transduction pathways are elusive, and results in the literature are even contradictory. Here, we examined the potential neuroprotective effect of GPR139 agonism in primary culture models of ... More