This product will be unavailable for purchase after April 1, 2016, or when stock is depleted, whichever comes first. We recommend Cat. No. XP00125BOX as an alternative. Learn about our Novex WedgeWell Tris-Glycine gels. Please contact us with any questions.

Precise™ 12% Tris-HEPES Gels, 5.8 cm x 8 cm x 1 mm, 15-Well

Catalog number: 25242

Thermo Scientific™  Related applications: Protein Gel Electrophoresis

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Thermo Scientific Precise Tris-HEPES Protein Gels, 12%, 15-Well are precast polyacrylamide gels for protein electrophoresis (PAGE) that use Tris-HEPES running buffer, have long shelf lives and provide compatibility with different commercial gel tanks.

Features of Precise Tris-HEPES Protein Gels:

High-performance—unique running buffer produces excellent separation and high-resolution protein bands
Long shelf life—up to 12-month stability ensures consistent performance for the duration of each package of gels
Fast—45-minute run-time provides results quickly; transfers efficiently to nitrocellulose and PVDF membranes in just 90 minutes; stains quickly coomassie and silver stains
Large-volume wells—innovative well design accommodates twice the sample volume of typical mini-gel cassette wells (10-well=50µL)
Compatible—cassettes (8.5 cm x 10 cm x 4.5 mm) fit many popular mini-gel tanks, and gels can be loaded using standard Laemmli sample loading buffers
Several convenient styles—choose from five gel densities and three well formats

Precise Gels are cast in a durable plastic cassette using a neutral-pH buffer that prevents polyacrylamide breakdown and results in a long shelf life. High-resolution staining and transfer of proteins is accomplished quickly on these 1mm thick mini gels. Gels are individually packaged in an easy-to-open plastic pouch and are ready to run with no comb or tape to remove. Following electrophoresis, the cassettes are easy to dismantle (no special tools required) without damaging the gel.

SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) is used to determine the approximate molecular weights of proteins with reference to the mobility of standard proteins on the same gel. Gradient gels provide the appropriate gel pore size, making molecular weight estimation more accurate by sharpening stained protein bands.

The most popular buffer system for SDS-PAGE is the Laemmli system [Nature 227, 680-686 (1970)]. This system, however, is limited by extended running times and gel instability from hydrolysis of polyacrylamide to acrylic acid in alkaline conditions. This chemical change alters gel conductivity, causing a constant change in protein migration patterns with time.

Polyacrylamide hydrolysis does not occur at neutral pH, and Thermo Scientific Precise Protein Gels are cast at pH 7, yielding a long shelf life and assured reproducibility of protein migration patterns. The extra stability of Precise Protein Gels is combined with a new Tris-HEPES running buffer to give speed and excellent resolution with the same size ranges as the Laemmli system. The buffer can be made according to the recipe provided in the instruction booklet or purchased premixed.

Related Products
Precise™ 12% Tris-HEPES Gels, 5.8 cm x 8 cm x 1 mm, 10-Well
Precise™ 12% Tris-HEPES Gels, 5.8 cm x 8 cm x 1 mm, 12-Well
For Research Use Only. Not for use in diagnostic procedures.


Wells: 15 wells
Gel Size: Mini (5.8 cm x 8 cm)
Gel Type: Tris-HEPES Gel
Quantity: 10 gels
Thickness: 1.0 mm
Well Format: 1D Well
Product Size: 10 gels (1 box)
Gel Percentage: 12 %
Separation Range: 20 kDa to 120 kDa
Mode of Separation: Molecular Weight

Contents & storage

One box contains 10 gels. Store in refrigerator (2–8°C). Do not freeze. Shelf life is 12 months.


Manuals & protocols