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Trypsin-EDTA (0.05%), phenol red This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number:  25300120

Related applications:

Mammalian Cell Culture

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Gibco® Trypsin-EDTA is made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength, trypsin is widely used for cell dissociation, routine cell culture passaging, and primary tissue dissociation. The trypsin concentration required for dissociation varies with cell type and experimental requirements. See the complete range of Gibco® trypsin solutions and recommended dissociation conditions.

We offer a variety of trypsin formulations and animal origin-free TrypLE™ reagent that feature:

Quality Testing
Documented Traceability
Dual-site cGMP Manufacturing

This trypsin solution is modified as follows:

• Phenol Red

The complete formulation is available.

Quality Testing
Gibco® Trypsin solutions are tested for pH, osmolality, sterility, and performance. In addition, prior to manufacturing, the raw materials are verified for e-beam irradiation and tested for endotoxin, PPV, PCV 1/2, mycoplasma, bacterial, fungal, and viral contamination, as well as multiple activity assays, ash analysis, and moisture analysis.

Documented Traceability
We can provide detailed documentation to meet your regulatory needs. Gibco® Trypsin information available includes lot traceability, animal origin certificates, lot analyses, irradiation certificates, a viral inactivation summary, and supply chain transparency.

cGMP Manufacturing and Quality System
Gibco® Trypsin is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable Gibco® Trypsin solution made in our Scotland facility (25300-096). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
For Research Use or Further Manufacturing. Not for use in diagnostic procedures.


Concentration: 1 X
Product Type: Cell Culture Dissociation Reagents
Sterility: Sterile
Chelators: EDTA
Classification: Animal Origin
pH Range: 7.2 - 8.0
Osmolality: 270 - 310 mOsm/kg
Tested For: In Vitro Bioassay
Product Line: Gibco™
Form: Liquid
Phenol Red Indicator: Phenol Red
Shelf Life: 24 Months
Green Features: Sustainable packaging
Quantity: 20 x 100mL
Reagent Type: Trypsin
Cell Type: Mammalian
Source: Animal Origin
Solution Type: Trypsin
pH: 7.1 to 8

Contents & storage

Storage conditions: -5°C to -20°C
Shipping conditions: Dry ice
Shelf life: 24 months from date of manufacture