Hypersil GOLD™ Peptide HPLC Column

Hypersil GOLD Peptide columns are particularly suited for:
• Biopharmaceutical peptide mapping and manufacturing control (where PTMs, deamidation, and hydrophilic peptides matter).
• LC-MS peptide workflows include digest analysis, proteomics, and glycopeptide variant monitoring.

You need to ensure that your sample is fully dissolved and free of particles (e.g., filter 0.2 µm or centrifuge) before injection. The column supports both FA- and TFA-containing mobile phases with excellent peak shape for peptides. For column equilibration, you need to flush new columns with at least 10 column volumes of the new mobile phase. Additionally, take into consideration that at higher pH (>8) and elevated temperature, column lifetime may be shortened.

Hypersil GOLD Peptide columns are unique in that they hold onto hydrophilic peptides, ensuring no critical data is missed. In other words, it has been engineered (via the packing material, ligand density and pore structure) to provide better retention of less-hydrophobic peptide species compared to generic RP columns, to reduce early elution or loss of critical peptides in a digest map.

The Hypersil GOLD Peptide column is built on 1.9 µm, 175 Å pore size spherical, fully porous ultrapure silica, specifically optimized for peptide separations. It provides consistent retention of all peptides—including hydrophilic peptides—and resolves post-translational modifications such as deamidation. Compared with many general-purpose C18 peptide columns, it offers higher peak capacity, better retention of hydrophilic peptides, and enhanced sequence coverage.