Thermo Scientific™

Pierce™ SATP (N-succinimidyl-S-acetylthiopropionate)

Catalog number: 26100
Thermo Scientific™

Pierce™ SATP (N-succinimidyl-S-acetylthiopropionate)

Catalog number: 26100
Catalog Number
26100
Unit Size
50 mg
Price (USD)
Price: 109.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
2610050 mg
Price: 109.00
Your Price:
-
Product Overview
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Thermo Scientific Pierce SATP is a long-chain (4.1 angstrom spacer arm) reagent for covalent modification of primary amines and addition of a protected yet exposable sulfhydryl group, enabling heterobifunctional crosslinking strategies.

Features of SATP:

• Adds a protected sulfhydryl that can be deprotected by hydroxylamine
• Allows long-term storage of the sulfhydryl-modified molecule
• Offers more steric freedom than SATA for the unmasked sulfhydryl group
• Forms cleavable disulfide bonds with other sulfhydryl-containing molecules
• Reacts with primary amines (e.g., lysine residues proteins) to form stable amide bonds
• Preserves protein activity with its mild, non-denaturing reaction cdonditions

SATP (N-succinimidyl-S-acetylthiopropionate) adds sulfhydryl groups to proteins and other amine-containing molecules in a protected form. The modified molecule can be stored indefinitely and treated with hydroxylamine to expose the labile sulfhydryl group when needed for conjugation reactions. SATP contains an N-hydroxysuccinimide (NHS) ester, which forms a stable, covalent amide bond with primary amines (i.e., lysine residues and the amino termini of proteins) and releases NHS as a by-product. De-protection (deacylation) to generate a free sulfhydryl is accomplished using hydroxylamine-HCl.

Sulfhydryl groups present on proteins, peptides and other compounds are important in protein chemistry/modification reactions. Frequently, thiols are unavailable or absent within the molecules of interest. Several reagents and techniques are available for introducing sulfhydryl groups or disulfides into proteins and peptides, including Traut's Reagent, variants of SPDP, and variants of SATA (e.g., SATP).

Related Products
Pierce™ SATA
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Chemical Reactivity
Amine
Label or Dye
Sulfhydryl Group
Label Type
Conjugating Groups
Product Line
Pierce™
Crosslinker Type
Heterobifunctional
Reactive Moiety
Active Ester, Succinimidyl Ester, NHS Ester
Spacer
Mid-Length
Form
Powder
Reagent Type
SATP
Quantity
50 mg
Solubility
DMF, DMSO
Product Type
Crosslinker

Contents & Storage

Upon receipt store desiccated at -20°C. Product is shipped at ambient temperature.