PageRuler™ Unstained Low Range Protein Ladder

Catalog number: 26632

Thermo Scientific™  Related applications: Protein Gel Electrophoresis

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Description

Thermo Scientific PageRuler Unstained Low Range Protein Ladder is a mixture of seven (7) purified proteins and peptides (3.4 to 100kDa) for use as size standards in peptide and small-protein gel electrophoresis.

Features of PageRuler Unstained Low Range Protein Ladder:

Size range—seven proteins and one peptide spanning 3.4 to 100kDa
Ready-to-use—supplied in a loading buffer for direct loading on gels; no need to boil
Sharp bands—excellent accuracy for detection in with coomassie or silver stains
Reference bands—25kDa band is stronger than the others for easy orientation
Tagged—each protein in the ladder contains an integral Strep-tag™ II Sequence
Quality tested—each lot evaluated by SDS-PAGE and Western blotting

The protein MW markers in this ladder resolve into clearly identifiable sharp bands when analyzed by SDS-PAGE and stained with coomassie dye or silver. The 100kDa protein band indicates the approximate position of large proteins and the 25kDa band is of greater intensity and serves as a reference band. Proteins can be detected on Western blots by staining with Ponceau S, coomassie blue or other protein stains. In addition, the ladder proteins (except for the 5 and 3.4kDa peptides) contain an integral Step-tag™ II Sequence and may be detected on Western blots using Strep-Tactin™ Conjugates. The PageRuler Unstained Broad Range Protein Ladder is supplied ready-to-use: no heating, dilution or addition of a reducing agent is required before use.

Includes:
• Proteins in 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 100 mM DTT, 1 mM NaN3, 0.01% bromophenol blue and 33% glycerol

Applications:
• Accurate protein sizing on SDS-polyacrylamide gels and Western blots.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Stain Type: Unstained
System: SDS-PAGE
Product Size: 2 x 250 µL

Contents & storage

Upon receipt store at -20°C. Product with an ice pack.

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Manuals & protocols

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