Thermo Scientific PageRuler Prestained NIR Protein Ladder is a mixture of 10 proteins (11 to 250 kDa) that are blue-stained and fluor-labeled for near-IR fluorescent visualization and protein sizing following electrophoresis. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.
Product features • Fluorescent—detect using 670 nm red laser or 700 nm channels with fluorescence imagers • Reference band—55 kDa band has greater intensity for easy orientation
Applications • Monitoring protein migration during SDS-polyacrylamide gel electrophoresis • Monitoring protein transfer onto membranes after western blotting • Sizing of proteins on NIR fluorescent SDS-polyacrylamide gels and western blots
Specifications
Detection Method
Colorimetric, NIR Fluorescence (700 nm)
Number of Markers
10
Product Type
Protein Ladder
Ready to Load
Yes
Size Range
11 to 250 kDa
Stain Type
1 color: Blue
Molecular Weight (g/mol)
250, 130, 95, 70, 55, 43, 34, 26, 15, 11 kDa
Product Line
PageRuler™
Quantity
8 x 250 µL
System Type
SDS-PAGE
Contents & Storage
Contents: eight vials of 250 µL each
Storage buffer: 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol