2'-Fluoro-iBu-G CEP is a phosphoramidite containing 5' DMT and the nucleoside guanosine (G) with 2'-Fluoro protection, for use in oligonucleotide synthesis. Located in the ideal oligo position, the highly electronegative fluorine (2'-Fluoro) modification adopts a more thermodynamically stable A-form helix on hybridization to its target. Showing improved affinity and some nuclease resistance (compared to 2'-OH), the 2'-F modification has been proven useful in antisense, siRNA, and aptamer applications. The 'standard grade' marking denotes a baseline level of purity verified with analytical methods, and proven to be suitable for most oligo synthesis requirements. This product is supplied in a 30-mL amber bottle with a 20-400 finish neck style, which is compatible with most Expedite synthesizers.
Features: • Full chemical name—5'-O-(4,4'-Dimethoxytrityl)-N2-isobutyryl-2'-fluoroguanosine-3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite • Molecular formula—C44H53FN7O8P • Base—G • Base Protection—iBu • Formula weight—857.4 g/mol • CAS number—144089-97-4 • HPLC purity—>98% • 31P NMR purity—≥98%
For Research Use Only. Not for use in diagnostic procedures.