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Thermo Scientific™

Phosphorylating Phosphoramidite, standard grade, serum vial bottle

Catalog number: 27-1794-04
Thermo Scientific™

Phosphorylating Phosphoramidite, standard grade, serum vial bottle

Catalog number: 27-1794-04
Catalog Number
27-1794-04
Unit Size
0.25 g
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
27-1794-040.25 g
-
Product Overview
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Phosphorylating phosphoramidite can be utilized to achieve phosphorylation of the 5' oligo terminus (also known as Phosphate-ON or Chemical Phosphorylation Reagent (CPR)). Chemical phosphorylation is a cost-effective substitute to enzymatic methods (via T4 kinase and ATP), allowing efficient introduction of terminal phosphate groups. Oligos with a 5'-phosphate are conducive to a number of various applications utilizing the ligation of oligos to each other, such as NGS adapters, cloning, and gene construction. This product is supplied in a 5 mL amber bottle with a serum vial neck style, which is compatible with most Applied Biosystems synthesizers and AKTA synthesizers of oligonucleotide synthesizers.

Features:
• Full chemical name—2-[2-(4,4-Dimethoxytrityloxy)ethylsulfonyl]ethyl-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
• Molecular formula—C34H45F3N2O7PS
• Formula weight—656.66 g/mol
• HPLC Purity—≥95.0%

For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment)
AKTA Synthesizers, Applied Biosystems Synthesizers
Product Type
Phosphorylating CEP
Nucleoside Type
None (Modifier)
Shipping Condition
Room Temperature
Purity
95%
Quantity
0.25 g
Reagent Type
Phosphoramidite
Grade
Standard
Nucleic Acid Type
None (Modifier)
Form
Powder

Contents & Storage

Store in freezer (at or below -20°C).

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