PAC-rA CEP is a phosphoramidite containing 5' DMT and the nucleoside adenosine (A) with Phenoxyacetyl (PAC) base protection, for use in oligonucleotide synthesis. The UltraMILD monomers - phenoxyacetyl (PAC)-dA, acetyl (Ac)-dC, and iso-propyl-phenoxyacetyl (iPr-PAC)-dG - were developed to alleviate the problem of modifiers and labels not withstanding prolonged exposure to strongly alkaline deprotection conditions. Cleavage and deprotection can be achieved in 4 hours at room temperature with 0.05M potassium carbonate in methanol. The TheraPure trademark denotes our highest level of phosphoramidite purity, developed with the greatest stringency of high definition analytics and tightly controlled impurity profiles and, therefore, ideal for any therapeutic application. This product is supplied in a 20 mL Amber bottle with a Serum Vial neck style, which is compatible with most ABI Systems|AKTA Systems of oligonucleotide synthesizers.
Features: • Full chemical name—5'-O-(4,4'-Dimethoxytrityl)-N6-phenoxyacetyl-2'-O-TBDMSi-adenosine-3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite • Molecular formula—C54H68N7O9PSi • Base—rA • Base Protection—PAC • Formula weight—1018.2 g/mol • CAS number—121058-86-4 • HPLC Purity—≥99% • 31P NMR Purity—≥99%; Sum of non-primary peaks in 140-152 ppm range: ≤0.5%; No single PIII impurity (140-152 ppm) >0.3%
For Research Use Only. Not for use in diagnostic procedures.
For Use With (Equipment)
AKTA Synthesizers, Applied Biosystems Synthesizers