EZ-Link™ Hydrazide-Biocytin

Catalog number: 28020

Thermo Scientific™  Related applications: Protein Labeling & Crosslinking

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Thermo Scientific EZ-Link Hydrazide-Biocytin is a mid-length, hydrazide-activated biotinylation reagent that contains an extra amine for greater reagent solubility during labeling reactions with carbonyl-containing compounds.

Features of EZ-Link Hydrazide-Biocytin:

Glycoprotein labeling—biotinylate glycosylated proteins at sialic acid residues for detection or purification using streptavidin probes or resins
Cell surface labeling—biotinylate and isolate cell surface glycoproteins; reagent does not permeate membranes of whole cells
Aldehyde-reactive—reacts with aldehydes formed by periodate-oxidation of sugar groups
Hydrazide-activated—perform reactions at pH 4 to 6 in buffers such as sodium acetate
Irreversible—forms semi-permanent hydrazone bonds; spacer arm cannot be cleaved
Solubility—usually dissolved in DMSO before further dilution in aqueous buffers
Medium length—spacer arm (total length added to target) is 19.7 angstroms; contains an additional primary amine, which can be targeted as a second conjugation point

Hydrazide-activated biocytin enables simple and efficient biotin labeling of polyclonal antibodies and other glycoproteins. Mild oxidation of sialic acid sugars with sodium periodate produces reactive aldehydes that can be conjugated to hydrazides. This compound is identical to Biotin-LC-Hydrazide (Part No. 21340), except that it possesses an additional primary amine in its spacer arm. This makes the reagent slightly more soluble and also makes possible other labeling and conjugation applications.

We manufacture biotin reagents to ensure the highest possible overall product integrity, consistency and performance for the intended research applications.

Biotinylation reagents differ in reactivity, length, solubility, cell permeability and cleavability. Hydrazides and alkoxyamines are two types of carbonyl-reactive groups. Hydrazides (—NH-NH2) react specifically with aldehyde groups in slightly acidic conditions to form hydrazone linkages; these can be further reduced to stable secondary amine bonds using sodium cyanoborohydride (Part No. 44892). The reaction is more efficient in the presence of aniline (Part No. 88944). Alternatively, hydrazides can be conjugated to carboxylic acids using EDC carbodiimide chemistry.

Reactive aldehyde groups can be generated in glycoproteins and other polysaccharide compounds by oxidation of constituent sugar diols using sodium periodiate (Part No. 20504). Sialic acid residues are common components of protein glycosylation and are easily converted to aldehydes with 1 mM NaIO4.

• Labeling erythrocyte membranes
• Labeling cytosine residues in DNA and RNA via a bisulfite-catalyzed transamination reaction
• Labeling of resolved glycoproteins on blots using either a galactose oxidase or a periodate oxidation method
For Research Use Only. Not for use in diagnostic procedures.


Spacer: Short
Cell Permeability: Cell-Permeant
Label or Dye: Biotin Analog
Chemical Reactivity: Carboxylic Acid, Aldehyde, Ketone
Solubility: DMSO (Dimethylsulfoxide), DMF (Dimethylformamide)
Product Size: 25 mg

Contents & storage

Store at room temperature.


Manuals & protocols