Thermo Scientific™

POROS™ CaptureSelect™ C1-InhXL Affinity Matrix

Catalog number: 2803402050
Have Questions?
Thermo Scientific™

POROS™ CaptureSelect™ C1-InhXL Affinity Matrix

Catalog number: 2803402050
Have Questions?
Catalog Number
2803402050
Unit Size
50 mL
Price (USD)
Availability
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Quantity
Catalog Number
Unit Size
5 mL
Price (USD)
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Catalog Number
Unit Size
10 mL
Price (USD)
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Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
280340205050 mL
-
28034020055 mL
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280340201010 mL
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POROS CaptureSelect C1-InhXL Affinity Matrix is developed for the purification of human C1 esterase inhibitor from recombinant and plasma/serum sources. C1 esterase inhibitor is a protease inhibitor belonging to the serpin superfamily that inhibits the spontaneous activation of the complement system. The CaptureSelect C1-InhXL affinity ligand is highly selective for human C1-inhibitor and coupled to the high-performing POROS matrix, allowing for an efficient and scalable purification process, with high purity and yield obtained in a single step. The large pore structure of the POROS beads enables higher flowrates and efficient binding of larger biomolecules.

Features of POROS CaptureSelect C1-InhXL Affinity Matrix include:

  • Efficient purification of C1 esterase inhibitor with high yield
  • Mild pH elution condition to retain activity
  • High binding capacity
  • Excellent scalability
  • Non-animal derived

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics:

  • Matrix: cross-linked poly(styrene-divinylbenzene)
  • Average particle size: 50 μm
  • Ligand: CaptureSelect C1-InhXL affinity ligand
  • Ligand coupling method: epoxide coupling
  • Binding capacity:∼3–5 g C1-inhibitor/L resin
  • Elution condition: 50 mM sodium acetate, 1.0 M MgCl2 , pH 5.0
  • Regeneration conditions: acidic stripping condition with 0.5 M acetic acid, pH 2
  • Mechanical resistance: 100 bar (1450 psi, 10 MPa)
  • Formulation buffer: 20% (v/v) ethanol

Specifications

Binding Capacity
∼3 to 5 g/L
Matrix
Cross-linked poly(styrene-divinylbenzene)
Particle Size
50 μm
Form
Resin Slurry
Product Line
CaptureSelect™
Quantity
50 mL
Type
Affinity Matrix
Ligand Type
C1-InhXL affinity ligand

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