Thermo Scientific™

POROS™ CaptureSelect™ AdV5 Affinity Matrix

Catalog number:  2803532005

Related applications:

Clinical & Translational Research

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Description

POROS CaptureSelect AdV5 Affinity Matrix was designed specifically for the purification and/or scavenging of recombinant hexon capsomeres of Adenovirus serotype 5 (AdV5) particles, as well as free hexon proteins thereof, for high purity in a single step. This affinity resin specifically binds AdV5 virus particles and enables a robust, fast, and efficient purification for research applications with excellent purity and yield in one step.

Features of this affinity matrix include:
• One-step purification of virus particles from crude material with high purity and yield
• High specificity, capacity, and retained activity
• Excellent scalability
• Non-animal derived
• High flow rates

Free of animal components
CaptureSelect products are affinity ligands created by a proprietary technology based on camelid-derived single-domain antibody fragments. The ligand is a 13-kDa fragment that comprises the three complementarity determining regions (CDRs) that form the antigen-binding domain, efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

View other viral vector and vaccine affinity matrix formats

Main characteristics:

Matrix: cross-linked poly(styrene-divinylbenzene)
Average particle size: 50 µm
Ligand: CaptureSelect AdV5 affinity ligand
Ligand coupling method: epoxide
Binding capacity: >1x1011 genome copies/mL of resin
Elution conditions: acidic elution with 0.1 M glycine, pH 3.0
Mechanical resistance: 100 bar (1,450 psi, 10 MPa)
Formulation buffer: 20% (v/v) ethanol

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Line: CaptureSelect™, POROS™
Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice
Format: Bottle(s)
Stationary Phase: CaptureSelect AdV5 affinity ligand

Contents & storage

Store in refrigerator (2–8°C).