POROS™ CaptureSelect™ AdV5 Affinity Matrix

POROS CaptureSelect AdV5 Affinity Matrix was designed specifically for the purification and/or scavenging of recombinant hexon capsomeres of Adenovirus serotype 5 (AdV5) particles, as well as free hexon proteins thereof, for high purity in a single step. This affinity resin specifically binds AdV5 virus particles and enables a robust, fast, and efficient purification for research applications with excellent purity and yield in one step.

Features of this affinity matrix include:

• One-step purification of virus particles from crude material with high purity and yield
• High specificity, capacity, and retained activity
• Excellent scalability
• Non-animal derived
• High flow rates

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics:

• Matrix: cross-linked poly(styrene-divinylbenzene)
• Average particle size: 50 μm
• Ligand: POROS CaptureSelect AdV5 affinity ligand
• Ligand coupling method: epoxide
• Binding capacity: >1 x 10¹¹ genome copies/mL of resin
• Elution conditions: acidic elution with 0.1 M glycine, pH 3.0
• Mechanical resistance: 100 bar (1450 psi, 10 MPa)
• Formulation buffer: 20% (v/v) ethanol