Thermo Scientific Pierce PMPI is a maleimide-and-isocyanate crosslinker for attaching compounds to sulfhydryl groups (cysteines) after conjugating the linker to hydroxyl groups on a molecule by urethane (carbamate) bonds.
Features of PMPI:
• Reactive groups:maleimide and isocyanate • Reactive towards: sulfhydryl and hydroxyl groups • Short (8.7A), sulfhydryl-to-hydroxyl crosslinker with an aromatic spacer arm (noncleavable) • Maleimide group reacts with sulfhydryl groups to form stable thioether linkages • Isocyanate reacts with non-aqueous hydroxyl groups to form carbamate linkage • Excellent tool for conjugating hydroxyl-containing compounds such as steroids and vitamins • Targets and reaction solutions must not contain primary amines
For Research Use Only. Not for use in diagnostic procedures.