Thermo Scientific™

PMPI (p-maleimidophenyl isocyanate)

Catalog number:  28100

Related applications:

Protein Labeling & Crosslinking

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Thermo Scientific Pierce PMPI is a maleimide-and-isocyanate crosslinker for attaching compounds to sulfhydryl groups (cysteines) after conjugating the linker to hydroxyl groups on a molecule by urethane (carbamate) bonds.

Features of PMPI:

Reactive groups: maleimide and isocyanate
Reactive towards: sulfhydryl and hydroxyl groups
• Short (8.7A), sulfhydryl-to-hydroxyl crosslinker with an aromatic spacer arm (noncleavable)
• Maleimide group reacts with sulfhydryl groups to form stable thioether linkages
• Isocyanate reacts with non-aqueous hydroxyl groups to form carbamate linkage
• Excellent tool for conjugating hydroxyl-containing compounds such as steroids and vitamins
• Targets and reaction solutions must not contain primary amines
For Research Use Only. Not for use in diagnostic procedures.


Product Line: Pierce™
Crosslinker Type: Heterobifunctional
Labeling Method: Chemical Labeling
Reactive Moiety: Maleimide, Isocyanate
Solubility: DMSO (Dimethylsulfoxide), DMF (Dimethylformamide)
Spacer: Short

Contents & storage

Upon receipt store at 4°C protected from moisture. Product is shipped at ambient temperature.