Thermo Scientific™

Pierce™ 10X Tris-Glycine SDS Buffer

Catalog number:  28362

Related applications:

Protein Electrophoresis & Western Blotting

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Description

Thermo Scientific Pierce 10X Tris-Glycine-SDS Buffer is a space-saving stock solution that is ideal for quickly preparing standard Tris-glycine-SDS (pH 8.3) running buffer used for SDS-PAGE (protein polyacrylamide gel electrophoresis).

Features of 10X Tris-Glycine-SDS Buffer:

Tris-glycine-SDS buffer—diluted 10-fold in water, the solution yields 0.025M Tris, 0.192M glycine, 0.1% SDS, pH 8.5
Easy to use—no packets to open and no powder to dissolve
Increased accuracy—eliminates the possibility of powder remaining in a packet
Saves time—10X concentration eliminates time spent waiting for powder to dissolve
Saves space—storage as concentrated stock minimizes bench space needed for solutions

Pierce Concentrated Buffers are ready to use without having to weigh and dissolve dry ingredients or to adjust the pH with concentrated acid or base. Simply dilute the stock solution with pure water and proceed with your experiment. The 10X Tris-Glycine-SDS Buffer makes 0.025M Tris, 0.192M glycine, 0.1% SDS, pH 8.5, when diluted to 1X with water. SDS is sodium dodecyl sulfate.

Applications:
• Running buffer for Tris-glycine gel electrophoresis

Related Products
BupH™ Tris-Glycine-SDS Buffer Packs
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Buffer: Running Buffers
Gel Compatibility: Novex® Tris-Glycine Gels, Tris-Glycine Gels, Precise™ Tris-Glycine Gels
Gel Type: Tris-Glycine Gel
Product Line: Pierce™
Concentration: 10 X

Contents & storage

Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 2 to 8°C.