Thermo Scientific™

Pierce™ 20X Tris-HEPES-SDS Buffer

Catalog number:  28368

 Related applications:

Protein Gel Electrophoresis

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Thermo Scientific Pierce 20X Tris-HEPES-SDS Buffer is a space-saving stock solution used for preparing the Tris-HEPES-SDS running buffer required for gel electrophoresis (SDS-PAGE) with Thermo Scientific Precise and Pierce Precast Gels.

Features of Thermo Scientific Pierce 20X Tris-HEPES-SDS Buffer:

Tris-HEPES-SDS buffer—diluted 20-fold in water, the solution yields 0.1M Tris, 0.1M HEPES, 3 mM SDS, pH 8
Easy to use—no packets to open and no powder to dissolve
Increased accuracy—eliminates the possibility of powder remaining in a packet
Saves time—20X concentration eliminates time spent waiting for powder to dissolve
Saves space—storage as concentrated stock minimizes bench space needed for solutions

• Running buffer for SDS-PAGE with Thermo Scientific Precise Precast Gels

Pierce Concentrated Buffers are ready to use without having to weigh and dissolve dry ingredients or to adjust the pH with concentrated acid or base. Simply dilute the stock solution with pure water and proceed with your experiment. The 20X Tris-HEPES-SDS Buffer makes 0.1M Tris, 0.1M HEPES, 3 mM SDS, pH 8, when diluted to 1X with water. HEPES is 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid.

Related Products
BupH™ Tris-HEPES-SDS Running Buffer
For Research Use Only. Not for use in diagnostic procedures.


Gel Type: Tris-HEPES Gel
Buffer Type: Running Buffers
Concentrated: 20 X
Gel Compatibility: Precise™ Tris-HEPES Gels
Product Line: Pierce™
Product Size: 500 mL

Contents & storage

Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 20 to 25°C.


Manuals & protocols