Thermo Scientific™

BupH™ Tris-Glycine Buffer Packs

Catalog number:  28380

 Related applications:

Protein Electrophoresis & Western Blotting

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Thermo Scientific BupH Tris-Glycine Transfer Buffer Packs are pouches of dry-blend powder that are each sufficient to make 500 mL of standard transfer buffer for wet or semi-dry electrophoretic protein transfer from gel to blotting membrane.

Features of Thermo Scientific BupH Tris-Glycine Transfer Buffer Packs:

Tris-glycine buffer—dissolved in 400 mL of water and 100 mL methanol, makes 25 mM Tris, 192 mM glycine, pH 8
Convenient—dissolve contents of one envelope in water and the buffer is ready to use
Save time and trouble—no weighing, no pH adjustment, no need to stock individual components, and no need to make and store large volumes of stock solution in advance of daily needs
Long shelf life—stocking and storage as dry packs eliminates concerns about long-term stability of stock solutions
Eliminate variables—our quality control ensures that every pack will yield the same, consistent buffer

• Running buffer for gel-to-membrane electrophoretic transfer

BupH Dry-Blend Packs of Tris-glycine buffer are easy to use. Simply empty contents of one foil envelope pack into a beaker, add ultrapure water and stir to dissolve. The packs eliminate weighing time and tedious pH adjustments. When dissolved in 400 mL of water and 100 mL methanol, makes 25 mM Tris, 192 mM glycine, pH 8.

Related Products
Pierce™ 10X Tris-Glycine Buffer
For Research Use Only. Not for use in diagnostic procedures.


Gel Type: SDS-PAGE Gel (any)
Buffer Type: Transfer Buffers
Concentrated: Dry Blend
Gel Compatibility: SDS-PAGE Gels
Product Line: BupH™
Product Size: 40 packs

Contents & storage

Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 20 to 25°C.


Manuals & protocols