Pierce™ Formic Acid, LC-MS Grade

Catalog number: 28905

Thermo Scientific™  Related applications: Protein Mass Spectrometry Analysis

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Description

Thermo Scientific Pierce Formic Acid is high-purity solvent supplied in bottles or ampules as a convenient, contamination-free alternative for preparing elution solvents for HPLC separations of protein and peptides. This product is packaged in amber glass, pre-scored, nitrogen-flushed ampules to protect formic acid from light, moisture and contamination.

Features of Formic Acid:

Greater than 99% pure formic acid—consistent LC baselines, no potential interference introduced in LC or MS applications, and no signal suppression in the mass spectrometer
Convenient format—ampule packaging simplifies the preparation of gradient and isocratic mobile phases containing 0.1% (v/v) formic acid in water or acetonitrile; the contents of a single vial in a final volume of 1 L of solvent yields a mobile phase of the most common formic acid concentration.

General properties of formic acid
• Molecular formula: HCOOH (CH2O2)
• Molecular weight: 46.02
• Density: 1.22 g/mL
• CAS number: 64-18-6
• Refractive index: 1.3701–1.3721(20°C)
• Flash point: 69°C
• Freezing point: 8°C

Specifications of Thermo Scientific Pierce Formic Acid
• Visual: Clear liquid, free of particulate matter
• Identity (IR): Must show only peaks characteristic for the compound
• Purity: > 99%
• Refractive index: 1.3701 to 1.3721 (20°C, 589 nm)

Pierce Formic Acid is sealed in amber glass ampules under a dry nitrogen atmosphere. A pre-measured aliquot of acid greatly simplifies preparation of liter quantities of mobile phases at the standard 0.1% formic acid concentration. The quality of this formic acid coupled with either glass-ampule bottle packaging provides reliability and convenience that adds value to both the chromatographic and MS results.

Pierce Formic Acid is specially purified and tested to the highest specifications to ensure the integrity of your data, maximize sensitivity in your assay and to prolong the life of your equipment.

Formic acid is a common component of reverse-phase mobile phases that provide protons for LC/MS analysis. The presence of a low concentration of formic acid in the mobile phase is also known to improve the peak shapes of the resulting separation. Unlike trifluoroacetic acid (TFA), formic acid is not an ion-pairing agent and it does not suppress MS ionization of polypeptides when used as a mobile-phase component.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Form: Liquid
Product Size: 10 x 1 mL
Reagent Type: Solvent (Pure)

Contents & storage

Store in original container protected from direct sunlight in a dry, cool and well-ventilated area.

Documents

Manuals & protocols