CaptureSelect KappaXP Affinity Matrix is the improved version of CaptureSelect KappaXL Affinity Matrix, with a significant increase in binding capacity for both whole kappa monoclonal antibodies and kappa Fab's. It enables efficient elution, even at pH 6.0, and demonstrates an improved stability for caustic cleaning agents. It is specific for human antibodies or antibody fragments, without cross-specificity to bovine antibody.
CaptureSelect KappaXP matrix recognizes the kappa light chain of human antibodies, enabling purification of all kappa-containing Ig’s, Fab, and Fab2 fragments directly from complex sources and feed streams.
CaptureSelect KappaXP Affinity Matrix features include:
• Purification of kappa monoclonal antibodies, Fab, and Fab2 fragments directly from complex source materials in a single step with high purity and yield
• Mild elution for Fab fragments and antibodies
• Human specific, no binding to bovine antibodies
• Excellent scalability
• Excellent pH stability up to 100 mM NaOH for limited exposure
The high selectivity and high yields obtained using CaptureSelect KappaXP Affinity Matrix enable a robust and efficient purification process with excellent purity in one step.View other antibody therapeutic affinity matrix formats ›Free of animal components
CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).Main characteristics:Matrix:
agarose-basedAverage particle size:
65 ± 10 µmLigand:
CaptureSelect KappaXP affinity ligandLigand coupling method:
epoxide immobilization of the ligandFab binding capacity:
20–30 g IgG Kappa-Fab/liter resin depending on flow rate, column height, and residence timeIgG binding capacity:
35–45 g IgG/liter resin depending on flow rate, column height, and residence timeElution conditions:
20 mM citric acid or acetic acid, pH 3–4; 100 mM Tris, 1.5 M MgCl2
, pH 6Flow characteristics:
150–300 cm/h (up to 2 bar)Formulation buffer:
20% (v/v) ethanol
For Research Use Only. Not for use in diagnostic procedures.