Thermo Scientific™

CaptureSelect™ KappaXP Affinity Matrix

Catalog number:  2943212010

 Related applications:

Chromatography

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Description

CaptureSelect KappaXP Affinity Matrix is an improved version of CaptureSelect KappaXL Affinity Matrix, specifically designed for higher binding capacity. The selectivity is the same: the ligand binds the constant domain of the human kappa light chain, but it features an increased binding capacity (up to 45 g IgG/L), and a wider elution range (full elution up to pH 6.0) compared to other affinity resins that bind the kappa light chain.

Features of this affinity matrix include:
• Single-step purification of Ig’s and Fab and Fab2 fragments directly from complex source materials
• Mild elution for Fab fragments and antibodies
• Human specific, with no binding to bovine antibodies
• Excellent scalability
• Non-animal-derived

The high selectivity and yield obtained using CaptureSelect KappaXP Affinity Matrix enables a robust and efficient purification process with excellent purity obtained in one step.

View other research-scale antibody affinity resins ›

Free of animal components
Our CaptureSelect products are affinity ligands created through a proprietary technology based on camelid-derived single-domain antibody fragments. The ligand is a 13 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

Main characteristics:

Matrix: agarose-based, epoxide activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect KappaXP affinity ligand
Ligand coupling method: epoxide coupling of the ligand
Fab binding capacity: 20–30 g Fab/L resin depending on flow rate, column height, and contact time
IgG binding capacity: 35–45 g IgG/L resin depending on flow rate, column height, and contact time
Elution conditions: 20 mM citric acid or acetic acid, pH 3–4; 100 mM Tris; 2.0 M MgCl2, pH 6
Flow characteristics: 150–300 cm/h (up to 2 bar)
Formulation buffer: 20%(v/v) ethanol

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: Agarose Bead
Immobilized Phase: KappaXP ligand
Product Line: CaptureSelect™
Product Size: 10 mL
Shipping Condition: Wet Ice

Contents & storage

Store in refrigerator (2–8°C).

Documents

Manuals & protocols