Thermo Scientific™

CaptureSelect™ Bovine IgA Affinity Matrix

Catalog number:  2943511010

 Related applications:

Chromatography

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Description

The CaptureSelect™ Bovine IgA Affinity Matrix has been designed specifically for the purification of bovine IgA from whey.

Features of this affinity matrix include:
• binds monomeric, dimeric, and secretory bovine IgA
• one-step purification from whey
• excellent scalability

Free of animal components
CaptureSelect affinity ligands are created using a proprietary technology based on camelid-derived single domain antibody fragments. The ligand is a 13 kDa single domain fragment comprising the 3 complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced by the yeast Saccharomyces cerevisiae in a process free of animal components.

Main characteristics:

Matrix: agarose-based, aldehyde-activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect Bovine IgA affinity ligand
Ligand coupling method: aldehyde coupling via NH2 residues of the ligand
Binding capacity: ~10 g/L resin
Elution conditions: 0.1 M citric acid pH 3.0 or 0.1M glycine pH 3.0
Flow characteristics: 100–150 cm/h
Formulation buffer: 20% (v/v) ethanol

Specifications

Format: Bottle(s)
Immobilized Phase: Anti Bovine IgA
Product Line: CaptureSelect™
Product Size: 10 mL

Contents & storage

Store at 2–8°C.

Documents

Manuals & protocols