CaptureSelect™ TSH Affinity Matrix

CaptureSelect TSH Affinity Matrix was specifically designed for the purification of thyroid-stimulating hormone (TSH) from recombinant sources.

Some features of CaptureSelect TSH Affinity Matrix include:

• Highly selective for thyroid-stimulating hormone with no affinity for the other gonadotropins (FSH, LH, and HCG)
• Enables efficient purification of recombinant TSH with high yields and retained activity
• Offers excellent scalability
• Non–animal-derived

The high selectivity and yields obtained using CaptureSelect TSH Affinity Matrix enable a robust and efficient purification process with excellent purity obtained in one step.

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics:

• Matrix: agarose bead
• Average particle size: 65 μm
• Ligand: CaptureSelect TSH affinity ligand
• Ligand coupling method: epoxide
• Binding capacity: >5 mg/mL of resin
• Elution conditions: 0.1 M citrate buffer, pH 3.0
• Flow characteristics: 50–200 cm/h (up to 2 bar)
• Formulation buffer: 20% (v/v) ethanol