Thermo Scientific™

CaptureSelect™ IgG-Fc (rabbit) Affinity Matrix

Catalog number:  2943642010

 Related applications:

Chromatography

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Description

CaptureSelect IgG-Fc (Rabbit) Affinity Matrix has been specifically designed for the purification of rabbit IgG from recombinant and plasma sources. The ligand on the resin targets the Fc region of rabbit IgG antibodies independent of the light chain subclass.

Features of this affinity matrix include:
• Highly selective for rabbit IgG
• No binding to mouse, rat, human, bovine, horse, sheep, and goat IgG
• Complex growth media with fetal calf or horse serum can be applied
• Non-animal-derived

The high selectivity and yields obtained using CaptureSelect IgG-Fc (Rabbit) Affinity Matrix enables a robust and efficient purification process with excellent purity obtained in one step.

View other research scale antibody affinity resins ›

Free of animal components
Our CaptureSelect products are affinity ligands created by a proprietary technology based on camelid-derived single domain antibody fragments. The ligand is a 13-kDa single domain fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

Main characteristics:

Matrix: agarose-based
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect IgG-Fc (rabbit) affinity ligand
Ligand coupling method: epoxide
Binding capacity: >15 mg rabbit IgG/mL resin
Elution conditions: 1 M glycine, pH 3.0, or 20 mM acetic or citric acid, pH 3.0
Flow characteristics: 50–150 cm/h (up to 1 bar)
Formulation buffer: 20%(v/v) ethanol

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Column Type: Agarose Resin
Format: Bottle(s)
Immobilized Phase: anti IgG-Fc (rabbit) ligand
Product Line: CaptureSelect™
Product Size: 10 mL
Shipping Condition: Wet Ice

Contents & storage

Store in refrigerator (2–8°C).

Documents

Manuals & protocols