CaptureSelect™ IgG-Fc (rabbit) Affinity Matrix

CaptureSelect IgG-Fc (Rabbit) Affinity Matrix has been specifically designed for the purification of rabbit IgG from recombinant and plasma sources. The ligand on the resin targets the Fc region of rabbit IgG antibodies independent of the light chain subclass.

Features of this affinity matrix include:

• Highly selective for rabbit IgG
• No binding to mouse, rat, human, bovine, horse, sheep, and goat IgG
• Complex growth media with fetal calf or horse serum can be applied
• Non-animal-derived

The high selectivity and yields obtained using CaptureSelect IgG-Fc (Rabbit) Affinity Matrix enables a robust and efficient purification process with excellent purity obtained in one step.

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics:

• Matrix: agarose-based
• Average particle size: 65 ± 10 μm
• Ligand: CaptureSelect IgG-Fc (rabbit) affinity ligand
• Ligand coupling method: epoxide
• Binding capacity: >15 mg rabbit IgG/mL resin
• Elution conditions: 1 M glycine, pH 3.0, or 20 mM acetic or citric acid, pH 3.0
• Flow characteristics: 50–200 cm/h (up to 2 bar)
• Formulation buffer: 20%(v/v) ethanol