Thermo Scientific™

CaptureSelect™ Factor X Affinity Matrix

Catalog number:  2943702010

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CaptureSelect Factor X Affinity Matrix was specifically designed for the purification of human Factor X from plasma and recombinant sources.

Features of the CaptureSelect Factor X Affinity Matrix include:
• Highly selective for human Factor X with no affinity for other human plasma proteins
• Binds both Factor X and Factor Xa in the presence of calcium
• Enables efficient purification of Factor X with high yields and retained activity
• Mild elution at neutral pH using EDTA
• Offers excellent scalability
• Non-animal-derived

Coagulation Factor X is a vitamin K-dependent, liver-produced serine protease that serves as the first enzyme in the coagulation cascade to form fibrin. It is a two-chain glycoprotein with a molecular weight of approximately 59 kDa. Factor X normally circulates in the plasma as an inactive molecule with activation of Factor X involved in both the intrinsic and extrinsic coagulation pathways.

View other biosimilars and recombinant protein affinity matrices ›

Free of animal components
CaptureSelect affinity ligands are created using a proprietary technology based on camelid-derived single-domain antibody fragments. The ligand is a 14-kDa fragment comprising the three complementarity determining regions (CDRs) that form the antigen binding domain. The ligand is efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

Main characteristics

Matrix: agarose-based
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect Factor X affinity ligand
Ligand coupling method: epoxide
Binding capacity: >10 mg/mL resin
Binding conditions: 50 mM Tris, 150 mM NaCl, 5 mM CaCl2, pH 7
Elution conditions: 50 mM Tris, 150 mM NaCl, 5 mM EDTA, pH 7
Flow characteristics: 50–150 cm/h (up to 2 bar)
Formulation buffer: 20% (v/v) ethanol

For Research Use Only. Not for use in diagnostic procedures.


Column Type: Agarose Resin
Format: Agarose Bead
Immobilized Phase: CaptureSelect Factor X Affinity Ligand
Product Line: CaptureSelect™
Product Size: 10 mL
Shipping Condition: Wet Ice

Contents & storage

Store in refrigerator (2–8°C).


Manuals & protocols