CaptureSelect™ Lenti VSVG Affinity Matrix

CaptureSelect Lenti VSVG Affinity Matrix has been designed specifically for the purification of VSV-G pseudotyped lentivirus particles.

Features of this affinity matrix include:

• Efficient and high-yield single-step purification of lentivirus particles from recombinant suspension cultivated harvest
• Gentle, neutral pH elution conditions to retain lentiviral activity
• Excellent scalability
• Non-animal derived

CaptureSelect Lenti VSVG Affinity Matrix offers an efficient and scalable purification method for VSV-G pseudotyped lentivirus particles, with a binding capacity of 1 x 10¹¹ total particles/mL resin. Elution can be performed using gentle elution conditions, which helps to retain infectivity of the enveloped virus particles.

Animal origin statement

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics:

• Matrix: agarose-based, epoxide activated
• Average particle size: 65 ± 10 μm
• Ligand: CaptureSelect Lenti VSVG affinity ligand
• Ligand coupling method: epoxide coupling
• Binding capacity: ∼1 x 10¹¹ total particles/mL matrix
• Elution conditions: 50 mM HEPES, 150 mM NaCl, 0.8 M arginine, pH 7.5
• Strip conditions: 50 mM sodium phosphate, pH 12
• Flow characteristics: 50–200 cm/h (up to 2 bar)
• Formulation buffer: 20% (v/v) ethanol