EZ-Link™ Psoralen-PEG3-Biotin

Catalog number: 29986

Thermo Scientific™  Related applications: Protein Labeling & Crosslinking

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Description

Thermo Scientific EZ-Link Psoralen-PEG3-Biotin enables biotinylation of DNA or RNA via UV-light-activated intercalation of the psoralen group with thymine- and other pyrimidine-containing bases to form a covalent bonds.

Features of EZ-Link Psoralen-PEG3-Biotin:

Biomolecular labeling—biotinylate DNA or RNA (approximately 1 biotin per 10 to 20 bases) without interfering with hybridization
Photo-reactive—covalent attachment occurs in the presence of UV light (~350nm) for 10 to 30 minutes
Psoralen-activated—reacts through a cyclo-addition mechanism with the 5,6 double bond in thymine- and other pyrimidine-containing bases
Pegylated—spacer arm contains a hydrophilic, 3-unit, polyethylene glycol (PEG) group
Enhances solubility—pegylation imparts water solubility to the biotinylated molecule, helping to prevent aggregation of biotinylated antibodies stored in solution
Long reach —spacer arm (total length added to target) is 36.9 angstroms, minimizing steric hindrance for binding interactions with streptavidin

Psoralen-PEG3-Biotin is a photoactivatable reagent for biotinylation of DNA or RNA. Upon activation with UV-Light (~350nm), the psoralen group reacts with thymine- and other pyrimidine-containing bases to form a covalent bond. The hydrophilic polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the biotinylated molecule, thus reducing aggregation of labeled proteins stored in solution. The PEG spacer arm also gives this reagent a long and flexible connection to minimize steric hindrance involved with binding to avidin molecules.

We manufacture biotin reagents to ensure the highest possible overall product integrity, consistency and performance for the intended research applications.

Applications:
• Selective labeling of cell surface proteins (Ref.1)
• Biotinylation of hyaluronan (Ref.2)
• Labeling of endotoxin for receptor binding studies (Ref.3)
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Spacer: Pegylated, Extra-Long
Cell Permeability: Cell-Impermeant
Solubility: DMSO (Dimethylsulfoxide), Water, DMF (Dimethylformamide)
Label or Dye: Biotin
Product Size: 5 mg
Chemical Reactivity: Light-Activated

Contents & storage

Store at 4°C protected from light and moisture. Shipped at ambient temperature.

Documents

Manuals & protocols