Thermo Scientific™

Dionex™ CarboPac™ PA1 IC Columns for Dual EGC Applications

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Industrial & Applied Science

For predictable high resolution separation of mono-, di-, and some oligosaccharides, use the Thermo Scientific™ Dionex™ CarboPac™ PA1 (1 mm) columns, when operating in Dual EGC mode on Thermo Scientific™ Dionex™ ICS-6000 HPIC™ systems equipped with Thermo Scientific™ Chromeleon™ CDS software. Dual EGC mode enables electrolytic generation of potassium methane sulfonate and potassium hydroxide gradients (KMSA/KOH) gradient eluent for carbohydrate analysis using a Dionex KOH EGC 400 KOH Eluent Generator Cartridge and a Dionex MSA EGC 400 MSA Eluent Generator Cartridge in series. Using the Dual EGC allows for an expanded set of samples for gradient separations of some oligosaccharides along with mono-, di-, and trisaccharide's using electrolytically generated potassium methane sulfonate and potassium hydroxide gradients with a combined concentration up to 200 mM. Many separations require only an isocratic separation. However, some groups of analytes will require a step or gradient elution. Retention of carbohydrates can be varied with eluent concentration, in some cases changing the elution order as the eluent concentration increases.

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Combined with pulsed amperometric detection (PAD), this anion-exchange column provides fast, reproducible separations with no need for derivatization. The 1 mm (standard/microbore) Dionex CarboPac PA1, 1 mm columns for Dual EGC contain the same resin as the proven 4 mm and 2 mm Dionex CarboPac PA1 columns, which are used for non-Dual EGC carbohydrate analysis.

Unique advantages of Dionex CarboPac PA1 Columns for Dual EGC

  • Predictable, high-resolution, separation of monosaccharide and disaccharides using electrolytically generated potassium methanesulfonate and potassium hydroxide
  • Rugged all-purpose column to determine monosaccharides and disaccharides in a variety of matrices
  • Column chemistry approved for use in a variety of official methods for the analysis of foods (AOAC Methods 995.13, 996.04, 997.08, 2000.11, 2000.17 and 2001.02)