DMEM, hoher Glukosegehalt, ohne Glutamin, ohne Phenolrot

Zitierungen und Referenzen (2)

Gibco™

DMEM, hoher Glukosegehalt, ohne Glutamin, ohne Phenolrot

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.Weitere Informationen

Katalognummer	Menge
31053028	500 mL

Katalognummer 31053028

Preis (EUR)

31,42

Menge:

500 mL

Customize this product

Preis (EUR)

31,42

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:

With	Without
• High Glucose	• L-glutamine
	• Phenol Red
	• Sodium Pyruvate
	• HEPES

The complete formulation is available.

Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

In-vitro-Diagnostik

Specifications

ZelllinieHeLa, 293, Cos-7 und PC-12

ZelltypPrimäre Fibroblasten, Neuronen, Gliazellen, HUVECs und glatte Muskelzellen

Konzentration1 X

Glucosekonzentration4500 mg/L

Fertigungsqualität, HerstellungsqualitätcGMP-compliant under the ISO 13485 standard

ProduktlinieGibco

ProdukttypDMEM (Dulbecco's Modified Eagle Medium)

Menge500 mL

Haltbarkeit12 Monate ab Herstellungsdatum

VersandbedingungRaumtemperatur

KlassifikationOhne Stoffe tierischen Ursprungs

FormFlüssig

Serum LevelStandard-Serumzugabe

SterilitätSteril gefiltert

Sterilization MethodSterile-filtered

Mit AdditivenHoher Glukosegehalt

Ohne AdditiveKein Glutamin, Keine HEPES, Kein Phenolrot, Ohne Natriumpyruvat

Unit SizeEach

Inhalt und Lagerung

Lagerbedingungen: 2 bis 8 °C, vor Licht schützen
Versandbedingungen: Haltbarkeit bei
Raumtemperatur: 12 Monate ab Herstellungsdatum

Häufig gestellte Fragen (FAQ)

Do you offer DMEM, high glucose without phenol red?

We recommend purchasing DMEM, high glucose, no glutamine, no phenol red (Cat. No. 31053028 or 31053036) and supplementing it with 4 mM glutamine and 1 mM sodium pyruvate.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the manganese concentration in DMEM? Do you offer manganese-free DMEM?

Manganese is not present in the formulation of our catalog DMEM media products.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I understand that some media are worse than others for fluorescence imaging. How do I choose?

Most media contain phenol red, which can quench fluorescent dyes in the visible wavelengths. Most media also contain autofluorescent components, such as riboflavin, which can reduce signal-to-background. We offer FluoroBrite DMEM and HEPES-based Live Cell Imaging Solution, which have been optimized for fluorescent imaging. We also offer a number of media without phenol red. But if none of these are reasonable options for your experiment, then we also offer BackDrop Background Suppressor ReadyProbes Reagent, which can be added to quench media autofluorescence.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Should I be concerned about phenol red in my media when labeling my live cells with fluorescent dyes?

Some cell types accumulate phenol red, and this can pose a problem in the use of many fluorescent probes. Phenol red can quench visible-wavelength dyes and, although phenol red is non-fluorescent, various impurities may be fluorescent. We have many phenol red-free media to choose from. Our Live Cell Imaging Solution (HEPES-based) and our FluoroBrite DMEM have been optimized to be phenol red-free as well as to be non-autofluorescent.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

View all DMEM, hoher Glukosegehalt, ohne Glutamin, ohne Phenolrot FAQs

Zitierungen und Referenzen (2)

Zitierungen und Referenzen

Abstract

Murine cerebrovascular cells as a cell culture model for cerebral amyloid angiopathy: isolation of smooth muscle and endothelial cells from mouse brain.

Authors:Gauthier SA, Sahoo S, Jung SS, Levy E

Journal:Methods Mol Biol

PubMed ID:22528096

'The use of murine cerebrovascular endothelial and smooth muscle cells has not been widely employed as a cell culture model for the investigation of cellular mechanisms involved in cerebral amyloid angiopathy (CAA). Difficulties in isolation and propagation of murine cerebrovascular cells and insufficient yields for molecular and cell culture studies ... More

Potentially novel candidate biomarkers for head and neck squamous cell carcinoma identified using an integrated cell line-based discovery strategy.

Authors:Sepiashvili L, Hui A, Ignatchenko V, Shi W, Su S, Xu W, Huang SH, O'Sullivan B, Waldron J, Irish JC, Perez-Ordonez B, Liu FF, Kislinger T

Journal:Mol Cell Proteomics

PubMed ID:22918226

Head and neck squamous cell carcinomas (HNSCC) can arise from the oral cavity, oropharynx, larynx or hypopharynx, and is the sixth leading cancer by incidence worldwide. The 5-year survival rate of HNSCC patients remains static at 40-60%. Hence, biomarkers which can improve detection of HNSCC or early recurrences should improve ... More