Thermo Scientific™

Pierce™ Alkaline Phosphatase

Catalog number: 31391
Thermo Scientific™

Pierce™ Alkaline Phosphatase

Catalog number: 31391
Catalog Number
31391
Unit Size
20 mg
Price (USD)
1,194.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
5 x 20 mg
Price (USD)
4,560.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
3139120 mg
1,194.00
Your Price:
***
313925 x 20 mg
4,560.00
Your Price:
***
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Additional Information
Recommendations
Recommendations
This purified calf intestinal alkaline phosphatase (CIP) is supplied in Tris buffer and 50% glycerol for use in protein research methods. The main applications for alkaline phosphatase in molecular biology and protein research are to remove 5'-phosphate groups from DNA or as a reporter system for immunoassays such as ELISA. For these latter methods, the enzyme is usually conjugated to specific primary or secondary antibodies and its activity is detected with a color-forming (or light-generating) substrate.

Features of Alkaline Phosphatase:

Purified form—ready to dilute and conjugate without prior dialysis
Concentrated—approximately 20 mg/mL (lot-specific value reported)
High specific activity—typically greater than 1600 units/mg (lot-specific value reported)
Tris buffered solution—supplied in 5 mM Tris, 5 mM magnesium chloride and 0.1 mM zinc chloride,
pH ∼7.0 in 50% glycerol

One unit equals the amount of protein required to hydrolyze one micromole of p-nitrophenyl phosphate (PNPP) per minute at 25°C in a glycine buffer, pH 9.6.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Form
Solid
For Use With (Application)
Protein Research
Product Line
Pierce™
Label or Dye
AP (Alkaline Phosphatase)
Quantity
20 mg

Contents & Storage

Store at 2–8°C.

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