This product is manufactured in our Grand Island, US facility and is intended for sale in North America, Latin America, and the Asia/Pacific region—higher freight charges will apply to orders shipped elsewhere. An equivalent product, manufactured in Paisley, Scotland, is recommended for our customers in Europe, Africa, and the Middle East. Click to view.

      Ham's F-12 Nutrient Mix, GlutaMAX™ Supplement This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

      Catalog number: 31765035

      Gibco™  Related applications: Mammalian Cell Culture

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      Description

      Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. We offer a variety of Gibco® F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

      This Ham's F-12 is modified as follows:

      With
      • GlutaMAX™
      • Phenol Red

      The complete formulation is available.

      Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-12 has led to a variety of improved formulations. Gibco® F-12 with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.

      cGMP Manufacturing and Quality System
      For supply chain continuity, we manufacture Gibco® F-12 at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.

      Gibco® F-12 contains no proteins or growth factors. Therefore, F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-12 uses a sodium bicarbonate buffer system (1.176 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
      For In Vitro Diagnostic Use.

      Specifications

      Form: Liquid
      Glutamine: GlutaMAX™-I
      HEPES Buffer: No HEPES
      Phenol Red Indicator: Phenol Red
      Product Line: Gibco®, GlutaMAX™
      Product Size: 500 mL
      Sodium Pyruvate Additive: Sodium Pyruvate
      Volume (Metric): 500 ml
      Green Features: Fewer resources used, Less waste
      Shipping Condition: Room Temperature

      Contents & storage

      Storage conditions: 2-8° C. Protect from light
      Shipping conditions: Ambient
      Shelf life: 12 months from date of manufacture

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