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Gibco™

RPMI 1640 Medium, powder

Catalog number: 31800022
Gibco™

RPMI 1640 Medium, powder

Catalog number: 31800022
Catalog Number
31800022
Unit Size
10 x 1 L
Price (USD)
Price: 57.25
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
50 L
Price (USD)
Price: 129.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
10 L
Price (USD)
Price: 38.83
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
3180002210 x 1 L
Price: 57.25
Your Price:
***
3180010550 L
Price: 129.00
Your Price:
***
3180008910 L
Price: 38.83
Your Price:
***
Product Overview
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Additional Information
Recommendations
RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This RPMI is modified as follows:
WithWithout
• L-glutamine• HEPES
• Phenol Red• Sodium Bicarbonate

The complete formulation is available.

Using RPMI
RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors; therefore, requiring supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH. Powder forms of Gibco™ cell culture media require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

cGMP manufacturing and quality system
RPMI 1640 Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical RPMI 1640 product made in our Scotland facility (51800-019). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Specifications

Product Type
RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Sterility
Sterile-filtered
Serum Supplementation
Standard Serum Supplementation
Shipping Condition
Room Temperature
Product Line
Gibco™
Form
Powder
Glutamine
L-Glutamine
Phenol Red Indicator
Phenol Red
Shelf Life
36 Months From Date of Manufacture
Quantity
10 x 1 L
Cell Type
Leukemic cells
With Additives
L-Glutamine, Phenol Red
Without Additives
HEPES, Sodium Bicarbonate
Sodium Pyruvate Additive
No Sodium Pyruvate
Cell Line
HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
Classification
Animal Origin-Free
Solution Type
RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
HEPES Buffer
No HEPES

Contents & Storage

Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture

Media Formulations