Gibco™

RPMI 1640 Medium, powder

Catalog number: 31800105
Gibco™

RPMI 1640 Medium, powder

Catalog number: 31800105
Catalog Number
31800105
also known as 31800-105
Unit Size
50 L
Price (USD)
Price: 143.00
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
31800105
also known as 31800-105
50 L
Price: 143.00
Your price:
-
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RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This RPMI is modified as follows:
WithWithout
• L-glutamine• HEPES
• Phenol Red• Sodium Bicarbonate


The complete formulation is available.

Using RPMI
RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors; therefore, requiring supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH. Powder forms of Gibco™ cell culture media require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

Specifications

Cell Line
HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
Cell Type
Leukemic Cells
Classification
Animal Origin-free
Form
Powder
Product Type
RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Serum Level
Standard Serum Supplementation
With Additives
Glutamine, Phenol Red
Without Additives
No HEPES, No Sodium Pyruvate, No Sodium Bicarbonate
Manufacturing Quality
cGMP-compliant under the ISO 13485 standard
Product Line
Gibco™
Quantity
50 L
Shipping Condition
Room Temperature

Contents & Storage

Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture

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