RPMI 1640 Medium, no glutamine
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RPMI 1640 Medium, no glutamine
Gibco™

RPMI 1640 Medium, no glutamine

Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as aRead more
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Catalog number 31870017
Price (USD)/ 100 mL
14.59
-
Add to cart
Quantity:
100 mL
Customize this product
Price (USD)/ 100 mL
14.59
Add to cart
RPMI 1640 Medium, no glutamine
Catalog number31870017
Price (USD)/ 100 mL
14.59
-
Add to cart

Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes and carcinomas. We offer a variety of Gibco™ RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This RPMI 1640 is modified as follows:
WithWithout
• Phenol Red• L-glutamine
 • HEPES


The complete formulation is available.

Gibco™ RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B12 and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.

Customers using Gibco™ RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Cell TypeMammalian Cells
Concentration1 X
Culture TypeMammalian Cell Culture
FormLiquid
Product TypeRPMI 1640
Serum LevelStandard Serum Supplementation
Shelf Life12 months from date of manufacture
SterilitySterile-filtered
With AdditivesPhenol Red
Without AdditivesNo Glutamine, No HEPES, No Sodium Pyruvate
Green FeaturesSustainable packaging
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™
Quantity100 mL
Shipping ConditionRoom Temperature
Unit Size100 mL
Contents & Storage
Store in refrigerator (2–8°C).