RPMI 1640 Medium, no glutamine

Catalog number: 31870017

RPMI 1640 Medium, no glutamine

Catalog number: 31870017
Catalog Number
Unit Size
100 ml
Price (USD)
Price: 13.86
Your Price:
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
31870017100 ml
Price: 13.86
Your Price:

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Product Overview
Citations & References
Additional Information
RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This RPMI is modified as follows:
• Phenol Red• HEPES
• L-glutamine

The complete formulation is available.

Using RPMI
RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Customers using Gibco™ RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

cGMP manufacturing and quality system
RPMI 1640 Medium is manufactured at a cGMP-compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical RPMI 1640 product made in our Grand Island facility (21870-092). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.


Cell Type
Mammalian Cells
1 X
Culture Type
Mammalian Cell Culture
Product Type
RPMI 1640
Serum Level
Standard Serum Supplementation
Shelf Life
12 months from date of manufacture
With Additives
Phenol Red
Without Additives
No Glutamine, No HEPES, No Sodium Pyruvate
Green Features
Sustainable packaging
Product Line
100 mL
Shipping Condition
Room Temperature

Contents & Storage

Store in refrigerator (2–8°C).