Zitierungen und Referenzen (3)

Gibco™

IMDM, GlutaMAX™ Supplement

IMDM (Iscove's Modified Dulbecco's Medium) is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells.Weitere Informationen

Katalognummer	Menge
31980030	500 mL

Katalognummer 31980030

Preis (EUR)

65,75

Menge:

500 mL

Customize this product

Preis (EUR)

65,75

IMDM (Iscove's Modified Dulbecco's Medium) is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells. We offer a variety of IMDM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This IMDM is modified as follows:

With: GlutaMAX™, Phenol Red

Without: α-thioglycerol, 2-mercaptoethanol

The complete formulation is available.

Using IMDM

IMDM, a modification of Dulbecco's Modified Eagle Medium, includes selenium as well as additional amino acids and vitamins. In addition, this unique medium lacks iron, with potassium nitrate replacing ferric nitrate. IMDM with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. IMDM contains no proteins, lipids, or growth factors. Therefore, IMDM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). IMDM uses a sodium bicarbonate buffer system (3.024 g/L), and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

In-vitro-Diagnostik

Specifications

ZelllinieJurkat, COS-7 und Makrophagen

Konzentration1 X

Fertigungsqualität, HerstellungsqualitätcGMP-compliant under the ISO 13485 standard

ProduktlinieGibco, GlutaMAX

ProdukttypIMDM (Iscove's Modified Dulbecco's Medium)

Menge500 mL

Haltbarkeit12 Monate ab Herstellungsdatum

VersandbedingungRaumtemperatur

KlassifikationOhne Stoffe tierischen Ursprungs

FormFlüssig

SterilitätSteril gefiltert

Sterilization MethodSterile-filtered

Mit AdditivenHoher Glukosegehalt, GlutaMAX, HEPES, Phenolrot, Natriumpyruvat

Ohne AdditiveKein α-Thioglycerol, Kein 2-Mercaptoethanol

Unit SizeEach

Inhalt und Lagerung

Lagerbedingungen: 2 bis 8 °C, vor Licht schützen
Versandbedingungen: Haltbarkeit bei
Raumtemperatur: 12 Monate ab Herstellungsdatum

Häufig gestellte Fragen (FAQ)

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

Will depletion, absence, or breakdown of essential growth-promoting components such as glutamine or growth factors reduce the growth rate of my culture?

Yes. If you suspect that this is the case, remove the medium and add fresh medium. Alternatively, you can supplement medium with growth-promoting components. It is also possible to substitute GlutaMax I or II for glutamine in the medium to prevent glutamine exhaustion.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Will the media formulations containing GlutaMAX supplement change with respect to L-glutamine content?

In all media containing GlutaMAX supplement dipeptides as a substitute for L-glutamine, concentration is equimolar with the L-glutamine in the original formulation.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all IMDM, GlutaMAX™ Supplement FAQs

Zitierungen und Referenzen (3)

Zitierungen und Referenzen

Abstract

Characterization of the laminin binding domains of the Lutheran blood group glycoprotein.

Authors:El Nemer W, Gane P, Colin Y, D'Ambrosio AM, Callebaut I, Cartron JP, Van Kim CL,

Journal:J Biol Chem

PubMed ID:11319237

'Lutheran (Lu) blood group antigens and the basal cell adhesion molecule antigen reside on two glycoproteins that belong to the Ig superfamily (IgSF) and carry five Ig-like extracellular domains. These glycoproteins act as widely expressed adhesion molecules and represent the unique receptors for laminin-10/11 in erythroid cells. Here, we report ... More

Specificity and affinity motifs for Grb2 SH2-ligand interactions.

Authors: Kessels Helmut W H G; Ward Alister C; Schumacher Ton N M;

Journal:Proc Natl Acad Sci U S A

PubMed ID:12084912

Protein-protein interactions are often mediated by the recognition of short continuous amino acid stretches on target proteins by specific binding domains. Affinity-based selection strategies have successfully been used to define recognition motifs for a large series of such protein domains. However, in many biological systems specificity of interaction may be ... More

Antigenic and Functional Properties of the Human Red Blood Cell Urea Transporter hUT-B1.

Authors: Lucien Nicole; Sidoux-Walter Frederic; Roudier Nathalie; Ripoche Pierre; Huet Martine; Trinh-Trang-Tan Marie-Marcelle; Cartron Jean-Pierre; Bailly Pascal;

Journal:J Biol Chem

PubMed ID:12093813

The Kidd (JK) blood group locus encodes the urea transporter hUT-B1, which is expressed on human red blood cells and other tissues. The common JK*A/JK*B blood group polymorphism is caused by a single nucleotide transition G838A changing Asp-280 to Asn-280 on the polypeptide, and transfection of erythroleukemic K562 cells with ... More