Thermo Scientific Pierce Antibody Extender Solution NC is a post-transfer nitrocellulose membrane-treatment reagent that enables Western blot detection with much less primary antibody.
Features of Antibody Extender Solution NC:
• Achieve equivalent signal—use at least three times less primary antibody than normal and retain comparable or better signal detectability
• Any detection mode or enzyme—the method works with both colorimetric and chemiluminescent detection modes and with horseradish peroxidase (HRP) and alkaline phosphatase (AP) systems
• Easy-to-perform, 10-minute protocol—invest a few minutes and save money on your primary antibody; our scientists have reduced their use of expensive primary antibodies by up to 100-fold with this product
By treating nitrocellulose membranes for a few minutes in this reagent immediately after transfer and before probing, you can use much less (more dilute) primary antibody solutions while obtaining the same level of detection. This conserves use of expensive and rare antibodies, enabling more experiments to be performed with the same amount of antibody. The reagent is so effective that our scientists have observed antibody cost reductions of 10-, 25- and even 100-times (depending on the antigen and primary antibody directed against that antigen).