Thermo Scientific™

Pierce™ ECL Western Blotting Substrate

Catalog number: 32209X4
Thermo Scientific™

Pierce™ ECL Western Blotting Substrate

Catalog number: 32209X4
Catalog Number
32209X4
Unit Size
4 x 250 mL
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
50 mL
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
250 mL
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
4 x 500 mL
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
500 mL
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
32209X44 x 250 mL
-
3210950 mL
-
32209250 mL
-
32106X44 x 500 mL
-
32106500 mL
-
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Thermo Scientific Pierce ECL Western Blotting Substrate is a value-priced, entry-level horseradish peroxidase (HRP) substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

Pierce ECL Plus Western Blotting Substrate characteristics include:
Sensitivity: low picogram
Stability: 1-hr working solution stability, 1-year kit stability at 4°C
Compatibility: nitrocellulose and PVDF membranes
Signal duration: 1 to 2 hours
Recommended primary antibody concentration: 1:1,000–1:5,000 dilution (0.2–10 μg/mL)
Recommended secondary antibody concentration: 1:1,000–1:15,000 dilution (0.07–1.0 μg/mL)

Compare and learn more about our western blot chemiluminescent substrates ›
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Pierce ECL Western Blotting Substrate provides reliability and performance equivalent to other standard ECL substrates for detection of HRP enzyme activity. It enables the detection of picogram amounts of antigen and allows for easy detection of HRP using photographic or other imaging methods.

Because the luminol and peroxide reagent formulations are identical to other commercially available substrate products, one can switch to Pierce ECL without needing to optimize probing conditions or incubation protocols. Pierce ECL Western Blotting Substrate provides performance identical to the original Amersham ECL Western Blotting Detection Reagent from GE Healthcare.

Specifications

Product Type
Substrate
Detection Method
Chemiluminescent
Form
Liquid
Product Line
Pierce™
Quantity
4 x 250 mL
Substrate
Horseradish Peroxidase
Recommended Antibody Concentrations
2°: 1:1-15 K (0.07-1 μg/mL)
1°: 1:1 K (0.2-10 μg/mL)
Sensitivity
Low picogram
Signal Duration
1 to 2 hr.

Contents & Storage

Upon receipt store at 2-8°C. Product is shipped at ambient temperature.

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