NHS-AMCA (succinimydyl-7-amino-4-methylcoumarin-3-acetic acid)

Catalog number:  33005

Thermo Scientific™  Related applications: Protein Labeling & Crosslinking

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}} also known as {{product.formattedSku}} 
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top


AMCA (aminomethylcoumarin acetate) is a blue fluorescent dye with reactive derivatives that are used as contrasting probes for double- and triple-labeling in immunofluorescence microscopy, arrays and in situ hybridization. The desirable properties of AMCA dyes include a relatively large Stoke's shift and resistance to photobleaching. NHS-AMCA and Sulfo-NHS-AMCA are reactive towards primary amine groups on proteins, peptides and other biomolecules. AMCA-Hydrazide is used to label glycosylation sites or for conjugation to carboxyl groups using the crosslinker EDC. AMCA has an excitation maximum at 345nm and an emission maximum at 450nm.

Features of NHS-AMCA:

AMCA —activated derivatives of blue-fluorescent aminomethylcoumarin acetate dye
Amine-specific labeling—NHS-ester and Sulfo-NHS-ester varieties of AMCA efficiently label antibodies and other purified proteins at primary amines (lysine side chains)
Glycoprotein labeling—hydrazide variety of AMCA labels oxidized sugar groups in glycoproteins or other carbohydrates

Related Products
Sulfo-NHS-AMCA (sulfosuccinimydyl-7-amino-4-methyl- coumarin-3-acetic acid)
Hydrazide-AMCA (hydrazide-activated aminomethylcoumarin acetate)
For Research Use Only. Not for use in diagnostic procedures.



LoyaltyEligible: SKUEligibleForLoyalty
LoyaltyTrialEligible: trialSkuFeature
Label or Dye: Methylcoumarin
Chemical Reactivity: Amine
Excitation⁄Emission (nm): 345-350⁄440-460
Product Line: Pierce™
Product Size: 5 mg
Solubility: DMF (Dimethylformamide)

Contents & storage

Store at 2–8°C.


Manuals & protocols