Thermo Scientific™

1-Step™ Chloronaphthol Substrate Solution

Catalog number:  34012

 Related applications:

Protein Electrophoresis & Western Blotting

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Thermo Scientific Pierce 1-Step Chloronaphthol Solution is a solution of 4-chloro-1-naphthol for the chromogenic detection of horseradish peroxidase (HRP) activity in Western blot and tissue staining methods.

Chloronaphthol (4-CN, MW = 178.6) reacts with HRP to form a distinct blue to blue-purple product to localize and visualize the activity of peroxidase-conjugated antibodies on blots or fixed tissue samples. The HRP substrate is not as sensitive or stable as other chromogenic substrates, such as TMB and DAB. However, the blue-purple precipitate photographs well, and its alcohol-solubility is a property that can be utilized in certain double-staining applications.

Features of Chloronaphthol Substrates:

HRP substrate—for detection of horseradish peroxidase activity on solid media
Chromogenic—no special equipment needed for visualization
Blue-purple—easy to photograph in color or black-and-white
Package options—choose powder or ready-to-use solution

Highlights of Pierce CN Solution
Ready-to-use—no ethanol or methanol required to dissolve; no dilution necessary for use
Complete—does not require buffer or addition of hydrogen peroxide
Pre-filtered—does not need to be filtered before use like powders or tablets after they are dissolved
Stable—store refrigerated at 4°C for at least one year

Related Products
Chloronaphthol Substrate Powder (4-chloro-1-naphthol)
WARNING: Reproductive Harm -
For Research Use Only. Not for use in diagnostic procedures.


Detection Method: Colorimetric
Product Line: 1-Step™
Product Size: 250 mL
Substrate Properties: Chemical Substrate
Substrate Type: HRP (Horseradish Peroxidase) Substrate

Contents & storage

Store at 4°C.


Manuals & protocols