Thermo Scientific™

ONPG (o-nitrophenyl-β-D-galactopyranoside)

Номер по каталогу: 34055
Thermo Scientific™

ONPG (o-nitrophenyl-β-D-galactopyranoside)

Номер по каталогу: 34055
Номер по каталогу
34055
Размер
5 g
Стоимость (RUB)
Номер по каталогуРазмерСтоимость (RUB)
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Рекомендации
Thermo Scientific Pierce ONPG is the preferred colorimetric substrate for ELISA applications involving beta-galactosidase (b-Gal) as the reporter enzyme.

When using beta-Galactosidase as the label for proteins in ELISA studies, a wide variety of substrates are available, including: o-Nitrophenyl-beta-D-galactopyranosidase (ONPG), Naphthol-AS-Bl-beta-D-galactopyranosidase (Nap-Gal), and 4-Methyl-umbelliferyl-beta-D-galactopyranosidase (MUm-Gal). However, it is important to choose a substrate with adequate solubility, that uses readily available equipment, and that gives a significant reading over the background. ONPG is a superior beta-Galactosidase substrate option.(1) The product formed is completely soluble and has a high extinction coefficient at 405nm. The substrate yields a yellow product that is easily detectable in the visual range after stopping the reaction with 1M Sodium Carbonate.
For Research Use Only. Not for use in diagnostic procedures.

Характеристики

Detection Method
Colorimetric
Product Type
ELISA Substrate
Substrate Properties
Chemical Substrate
Substrate Type
Beta-Gal Substrate

Содержимое и хранение

Upon receipt store at -20°C protected from moisture and light. Product is shipped at ambient temperature.