Thermo Scientific™

ONPG (o-nitrophenyl-β-D-galactopyranoside)

Catalog number:  34055

Related applications:

Protein Biology

Back to top

Description

Thermo Scientific Pierce ONPG is the preferred colorimetric substrate for ELISA applications involving beta-galactosidase (b-Gal) as the reporter enzyme.

When using beta-Galactosidase as the label for proteins in ELISA studies, a wide variety of substrates are available, including: o-Nitrophenyl-beta-D-galactopyranosidase (ONPG), Naphthol-AS-Bl-beta-D-galactopyranosidase (Nap-Gal), and 4-Methyl-umbelliferyl-beta-D-galactopyranosidase (MUm-Gal). However, it is important to choose a substrate with adequate solubility, that uses readily available equipment, and that gives a significant reading over the background. ONPG is a superior beta-Galactosidase substrate option.(1) The product formed is completely soluble and has a high extinction coefficient at 405nm. The substrate yields a yellow product that is easily detectable in the visual range after stopping the reaction with 1M Sodium Carbonate.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Substrate Properties: Chemical Substrate
Substrate Type: Beta-Gal Substrate
Detection Method: Colorimetric

Contents & storage

Upon receipt store at -20°C protected from moisture and light. Product is shipped at ambient temperature.