Metal Enhanced DAB Substrate Kit
Metal Enhanced DAB Substrate Kit
Thermo Scientific™

Metal Enhanced DAB Substrate Kit

Thermo Scientific Pierce Metal-Enhanced DAB Substrate Kit uses cobalt and nickel chloride in a special formulation of diaminobenzidine peroxidase substrateRead more
Have Questions?
Catalog NumberQuantity
34065275 mL of 1X working solution
Catalog number 34065
Price (USD)
207.65
Online Exclusive
235.00
Save 27.35 (12%)
Each
Add to cart
Quantity:
275 mL of 1X working solution
Request bulk or custom format
Price (USD)
207.65
Online Exclusive
235.00
Save 27.35 (12%)
Each
Add to cart
Thermo Scientific Pierce Metal-Enhanced DAB Substrate Kit uses cobalt and nickel chloride in a special formulation of diaminobenzidine peroxidase substrate that yields intense color for immunohistochemical staining.

Features of the Metal-Enhanced DAB Substrate Kit :

Incredible sensitivity—fifty times more sensitive than the traditional DAB method and thiry times more sensitive than other metal-intensified versions of DAB
Low background, high intensity—get a crisp dark brown-black precipitate that is more intense than the dull brown precipitate when using DAB without enhancement. And, even with the increased intensity, background is almost nonexistent
Only two components—simply mix the two liquid components and your working solution is ready to use
Six-hour stability—the innovative working solution is stable for more than six hours at room temperature, while other DAB substrates must be used immediately

To prepare the product, the 10X Metal Enhanced Substrate Solution is diluted with the Pierce Stable Peroxide Buffer prior to use, and the special formulation allows the 1X substrate to be stable for at least 6 hours at room temperature. The background color of the substrate does not increase and, unlike typical metal enhanced DAB methods, the reactivity is unchanged (1,2). The resulting precipate is a dark brown-black that is more intense than the brown color from DAB alone. The intensity not only increases, but the background is also reduced, which increases the signal-to-noise ratio and makes screening tissue or membrane antigens easier.
WARNING: Cancer – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodChromogenic, Colorimetric
For Use With (Application)Immunodetection, IHC (Immunohistochemistry)
Product TypeDAB Substrate Kit
Quantity275 mL of 1X working solution
SubstrateDAB (Enhanced)
Substrate TypeHRP (Horseradish Peroxidase) Substrate
Product LinePierce™
Unit SizeEach
Contents & Storage
  • Metal Enhanced DAB (10X), 25 mL (store at -20°C)
  • Stable Peroxide Buffer, 250 mL (store at 4°C)