Thermo Scientific™

SuperSignal™ West Femto Maximum Sensitivity Substrate

Catalog number:  34095

 Related applications:

Protein Assays and Analysis

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Thermo Scientific SuperSignal West Femto Maximum Sensitivity Substrate is an ultra-sensitive enhanced chemiluminescent (ECL) substrate for low-femtogram-level detection by Western blot analysis with horseradish peroxidase (HRP) enzyme.

Features of SuperSignal West Femto Maximum Sensitivity Substrate:

ECL —enhanced chemiluminescent substrate for horseradish peroxidase (HRP)
Sensitive—detect low-femtogram (mid-zeptomole) amounts of protein in bands on nitrocellulose or PVDF membranes when probed with appropriate primary and secondary antibodies
Quantitative—produces quantitative signal that is measurable over two orders of magnitude
Intense signal—easy to capture an image by exposure to film or imaging system
Excellent signal duration—8 hours of usable light-output when conditions are optimized
Stable reagent—kit components are stable for 1 year at 4°C or 6 months at room temperature
Economical—optimized for extremely dilute antibody concentrations:
• 1ng to 0.2 µg/mL primary antibody (1:5000 to 1:100,000 dilution from 1 mg/mL stock)
• 2 to 10 ng/mL secondary antibody (1:100,000 to 1:500,000 dilution from 1 mg/mL stock)

When combined with optimized antibody concentrations and blocking buffers, SuperSignal West Femto Substrate enables detection of target proteins in amounts that are too small to be seen with typical ECL substrates.
For Research Use Only. Not for use in diagnostic procedures.


Detection Method: Chemiluminescent
Product Line: SuperSignal™
Product Size: 100 mL
Substrate Properties: Chemical Substrate
Substrate Type: HRP (Horseradish Peroxidase) Substrate

Contents & storage

Sufficient For: 12 mini-gel blots; 1000 cm2 of membrane
• Luminol/Enhancer, 50 mL
• Stable Peroxide Buffer, 50 mL

Store reagents at 4°C.


Manuals & protocols