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SuperSignal™ West Femto Maximum Sensitivity Substrat
Zitierungen und Referenzen (1)
Thermo Scientific™

SuperSignal™ West Femto Maximum Sensitivity Substrat

Thermo Scientific SuperSignal West Femto Substrat mit maximaler Empfindlichkeit ist ein extrem empfindliches Substrat mit erhöhter Chemilumineszent (ECL) für niedrigeWeitere Informationen
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KatalognummerMenge
34096200 ml
3409420 ml
34095X44 x 100 ml
34095100 ml
34096X44 x 200 ml
Katalognummer 34096
Preis (EUR)
818,65
Exklusiv online
930,00
Ersparnis 111,35 (12%)
Each
Menge:
200 ml
Großbestellung oder individuelle Größe anfordern
Preis (EUR)
818,65
Exklusiv online
930,00
Ersparnis 111,35 (12%)
Each

Thermo Scientific SuperSignal West Femto Substrat mit maximaler Empfindlichkeit ist ein extrem empfindliches Substrat mit erhöhter Chemilumineszent (ECL) für niedrige Detektionsgrenzen im Femtogrammbereich durch Western-Blot-Analysen mit Meerrettich-Peroxidase-Enzymen (HRP).

Eigenschaften des SuperSignal West Femto Maximum Sensitivity Substrats:
• Empfindlichkeit: niedriger Femtogrammbereich
• Stabilität: 8-Stunden-Stabilität der Arbeitslösung, 1-Jahres-Stabilität des Kits bei 4 °C
• Kompatibilität: Nitrozellulose- und PVDF-Membranen
• Signaldauer: bis zu 8 Stunden
• Empfohlene Konzentration des primären Antikörpers: Verdünnung von 1:5.000–1:100.000 (10–200 ng/ml)
• Empfohlene Konzentration des Sekundärantikörpers: Verdünnung 1:100.000–1:500.000 (2–10 ng/ml)

Vergleichen Sie und erfahren Sie mehr über unsere Western-Blot-Chemolumineszenz-Substrate ›
Alle erhältlichen HRP-Substrate anzeigen›

SuperSignal West Femto Maximum Sensitivity Substrate wurde für die Detektion niedriger Femtogrammkonzentrationen durch Western-Blot-Analyse entwickelt. In Kombination mit optimierten Antikörperkonzentrationen und Blockierpuffern ermöglicht SuperSignal West Femto Substrat die Detektion von Zielproteinen in Mengen, die zu klein sind, um sie mit herkömmlichen ECL-Substraten zu erkennen.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
KonjugatHRP (Meerrettich-Peroxidase)
KitinhaltStabiler Peroxidpuffer, 100 ml, Luminol/Verstärker, 100 ml
Empfohlene Antikörperkonzentrationen2°: 1:100 bis 500 K (2 bis 10ng/ml), 1°: 1:5 K (10 bis 200 ng/ml)
EmpfindlichkeitNiedriger Femtogrammbereich
Signaldauer6 bis 8 h
Ausreichend für25 Mini-Gel-Blots, 2.000 cm2 Membran
NachweisverfahrenChemolumineszenz
Zur Verwendung mit (Anwendung)Western Blot
FormFlüssig
Membrane CompatibilityNitrozellulose, PVDF
ProduktlinieSuperSignal
ProdukttypSubstrat
Menge200 ml
SubstrateMeerrettich-Peroxidase
Unit SizeEach
Inhalt und Lagerung
Ausreichend für: 25 Mini-Gel-Blots; 2000 cm2 Membran
• Luminol/Enhancer, 100 ml
• stabiler Peroxidpuffer, 100 ml

Reagenzien bei 4 °C lagern.

Häufig gestellte Fragen (FAQ)

What types of membrane work best with SuperSignal West Femto Maximum Sensitivity Substrate?

Most researchers use nitrocellulose or polyvinyldiflouride (PVDF) membranes with SuperSignal West Femto Maximum Sensitivity Substrate. Both work well, although nitrocellulose seems to be better suited in some applications than PVDF. In addition, charge-modified nylon membrane performs well with this substrate. Please also see our guide for choosing western blot membranes (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/transfer-proteins-western-blot/membranes-transfer-buffers-western-blotting/membranes-western-blotting.html).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Will SuperSignal West Femto Maximum Sensitivity Substrate work with nucleic acid blots (Southern blotting, etc.)?

Yes. However, SuperSignal West Femto Maximum Sensitivity Substrate was optimized for use in Western blot sand is generally not sensitive enough for most nucleic acid protocols. For Southern and Northern blotting, use our North2South Chemiluminescent Nucleic Acid Hybridization and Detection Kit (Cat. No. 17097), North2South Chemiluminescent Substrate for HRP (Cat. No. 17295), or the Chemiluminescent Nucleic Acid Detection Kit, if probing for biotinylated probes (Cat. No. 89880).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

I used the SuperSignal West Femto Maximum Sensitivity Substrate. Why is the entire film black or showing a reversed image?

The antibody concentration (primary and secondary) is too high. Use the antibody dilutions described in the product instructions. Most background will disappear when the proper blocking reagent and a HRP conjugate concentration are used.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Why do I see more bands with the SuperSignal West Femto Maximum Sensitivity Substrate than I've seen in the past with other substrates?

Because this substrate is more sensitive than other chemiluminescent substrates you might detect low-abundance proteins that were not visible before. When using a more sensitive substrate, more careful optimization of blocking buffer steps and antibody concentrations is required.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What protocol should I use to strip and reprobe a blot detected with SuperSignal West Femto Maximum Sensitivity Substrate ?

See Tech Tip # 23: Strip and reprobe Western blots.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

View all SuperSignal™ West Femto Substrat mit maximaler Empfindlichkeit FAQs

Zitierungen und Referenzen (1)

Zitierungen und Referenzen
Abstract
Natural IgG autoantibodies are abundant and ubiquitous in human sera, and their number is influenced by age, gender, and disease.
Authors:Nagele EP, Han M, Acharya NK, DeMarshall C, Kosciuk MC, Nagele RG
Journal:PLoS One
PubMed ID:23589757
The presence of self-reactive IgG autoantibodies in human sera is largely thought to represent a breakdown in central tolerance and is typically regarded as a harbinger of autoimmune pathology. In the present study, immune-response profiling of human serum from 166 individuals via human protein microarrays demonstrates that IgG autoantibodies are ... More