Thermo Scientific™

SuperSignal™ West Femto Maximum Sensitivity Substrate

Catalog number:  34096

Related applications:

Protein Electrophoresis & Western Blotting

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Thermo Scientific SuperSignal West Femto Maximum Sensitivity Substrate is an ultra-sensitive enhanced chemiluminescent (ECL) substrate for low femtogram protein level detection by western blot analysis with horseradish peroxidase (HRP) enzyme.

SuperSignal West Femto Maximum Sensitivity Substrate characteristics include:
Sensitivity: low-femtogram (mid-zeptomole)
Stability: 8-hr working solution stability, 1-year kit stability at 4°C
Compatibility: nitrocellulose and PVDF membranes
Signal duration: up to 8 hours
Recommended primary antibody concentration: 1:5,000–1:100,000 dilution (10–200 ng/mL)
Recommended secondary antibody concentration: 1:100,000–1:500,000 dilution (2–10 ng/mL)

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SuperSignal West Femto Maximum Sensitivity Substrate is designed for low femtogram-level detection by western blot analysis. When combined with optimized antibody concentrations and blocking buffers, SuperSignal West Femto substrate enables detection of target proteins in amounts that are too small to be seen with typical ECL substrates.

WARNING: Cancer –
For Research Use Only. Not for use in diagnostic procedures.


Detection Method: Chemiluminescent
Product Line: SuperSignal™
Product Size: 200 mL
Recommended Antibody Concentrations: 2°: 1:100-500K (2-10ng/mL),1°: 1:5K (10- 200ng/mL)
Sensitivity: Low to mid-femtogram
Signal Duration: 6 to 8 hr
Substrate Properties: Chemical Substrate
Substrate Type: HRP (Horseradish Peroxidase) Substrate

Contents & storage

Sufficient For: 25 mini-gel blots; 2000 cm2 of membrane
• Luminol/Enhancer, 100 mL
• Stable Peroxide Buffer, 100 mL

Store reagents at 4°C.